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Defining the common subtypes of HLA A9, A10, A28 and A19 by use of ARMS/PCR.

作者信息

Krausa P, Bodmer J G, Browning M J

机构信息

Cancer Immunology Laboratory, Imperial Cancer Research Fund, John Radcliffe Hospital, Oxford, United Kingdom.

出版信息

Tissue Antigens. 1993 Aug;42(2):91-9. doi: 10.1111/j.1399-0039.1993.tb02243.x.

DOI:10.1111/j.1399-0039.1993.tb02243.x
PMID:8266322
Abstract

We describe sequence-specific primer (SSP) combinations for use in a one-step polymerase chain reaction (PCR) typing system to determine HLA-A locus subtypes of A9 (A23, A24), A10 (A25, A26, A43), A28 (A6801, A6802, A6901) and A19 (A2901, A2902, A3001, A*3002, A31, A32, A33) from genomic DNA. SSP's were designed on the basis of the amplification refractory mutation system (ARMS) in which a mismatch at the 3' residue inhibits non-specific amplification. The SSP combinations described extend our low-resolution typing system, to provide a high-definition typing of the HLA-A locus.

摘要

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