Bhargava H N
Psychopharmacology (Berl). 1976 Sep 29;49(3):267-70. doi: 10.1007/BF00426828.
Mice were rendered morphine-dependent by the subcutaneous implantation of a pellet containing 75 mg of morphine base; 72 h after the implantation, the animals were injected intraperitoneally either with vehicle or with various doses of delta9-tetrahydrocannabinol, delta8-tetrahydrocannabinol, cannabidiol, cannabinol, or 11-hydroxy-delta8-tetrahydrocannabinol. Thirty minutes after injection of the cannabinoids, the antagonist, naloxone HC1, was administered to induce the stereotyped withdrawal jumping syndrome. The dose of naloxone needed to induce withdrawal jumping in 50% of the animals (ED50) was determined for each dose of the cannabinoids. All of the cannabinoids inhibited the naloxone-precipitated morphine abstinence as evidenced by an increase in the naloxone ED50. Two additional signs of morphine abstinence, defecation and rearing behavior, were also suppressed by the cannabinoids. The relative effectiveness of the cannabinoids in inhibiting morphine abstinence appeared to be in the following order: delta9-tetrahydrocannabinol greater than delta8-tetrahydrocannabinol greater than 11-hydroxy-delta8-tetrahydrocannabinol greater than cannabidiol greater than cannabinol. These data suggest that cannabinoids may be useful in facilitating narcotic detoxification.
通过皮下植入含75毫克吗啡碱的药丸使小鼠产生吗啡依赖性；植入后72小时，给动物腹腔注射溶剂或不同剂量的Δ9-四氢大麻酚、Δ8-四氢大麻酚、大麻二酚、大麻酚或11-羟基-Δ8-四氢大麻酚。注射大麻素30分钟后，给予拮抗剂盐酸纳洛酮以诱发刻板性戒断跳跃综合征。针对每种剂量的大麻素，确定在50%的动物中诱发戒断跳跃所需的纳洛酮剂量（半数有效剂量[ED50]）。所有大麻素均抑制纳洛酮诱发的吗啡戒断，纳洛酮ED50增加即证明了这一点。大麻素还抑制了吗啡戒断的另外两个迹象，即排便和竖毛行为。大麻素抑制吗啡戒断的相对效力似乎按以下顺序排列：Δ9-四氢大麻酚＞Δ8-四氢大麻酚＞11-羟基-Δ8-四氢大麻酚＞大麻二酚＞大麻酚。这些数据表明，大麻素可能有助于促进麻醉剂解毒。
