Corcuff P, Bertrand C, Leveque J L
L'Oreal, Laboratoires de Recherche Avancée, Aulnay sous Bois, France.
Arch Dermatol Res. 1993;285(8):475-81. doi: 10.1007/BF00376820.
Real-time confocal microscopy has brought substantial improvements to the imaging of the human skin in vivo. On early images, the stratum corneum could be distinguished from the living epidermis and the circulatory network of the superficial dermis. We have adapted the Tandem Scanning Microscope to obtain images of the living skin, showing thinner structures such as the stratum lucidum and the dermo-epidermal junction, both of which are essential markers for micron-order measurements of the thickness of the stratum corneum and living epidermis. The measurements were corrected for the differences in the refractive index of the various cutaneous layers, and the undulation of the dermo-epidermal junction. Furthermore, nucleus size and number could be assessed from horizontal optical sections. To illustrate the sensitivity of the thickness measurements, changes in the thickness of the epidermis were recorded during and after stripping of the horny layers. This non-invasive methodology is a very promising tool for morphometric studies of the living human skin at the cellular level.
实时共聚焦显微镜技术为人体皮肤的体内成像带来了显著改进。在早期图像中，角质层可与活表皮及浅表真皮的循环网络区分开来。我们对串联扫描显微镜进行了改进，以获取活皮肤的图像，显示出诸如透明层和真皮 - 表皮连接等较薄结构，这两者都是微米级测量角质层和活表皮厚度的重要标志。针对各皮肤层折射率的差异以及真皮 - 表皮连接的起伏对测量进行了校正。此外，可从水平光学切片评估细胞核的大小和数量。为说明厚度测量的敏感性，在角质层剥离期间及之后记录了表皮厚度的变化。这种非侵入性方法是在细胞水平对活人皮肤进行形态计量学研究的非常有前景的工具。
