Castro-Córdoba F, Arenas-Huertero F, Salazar-Flores M, Osornio-Vargas A
Division of Basic Research, Instituto Nacional de Cancerología, México, D.F.
Arch Med Res. 1993 Summer;24(2):199-201.
Smith and Naylor's technique has been successfully used by many authors around the world to recover inorganic particles from the lungs. In this short report we compare the results of counting inorganic particles recovered from human lung tissue by two different methods: Smith and Naylor's technique and a modification to it. We used ferruginous bodies (FB) as markers for inorganic particles and we compared the results of the FB counts in both methods. In the traditional technique the interface formed in the 1:1 ethanol-chloroform mixture is discharged and FB are counted only in the formed pellet. Our modification also quantified FB in the interface fraction. Post-mortem samples from each of the lung lobes were taken from 22 individuals, totalling 198 samples. Each sample was digested in liquid commercial bleach and processed by both techniques. Our results showed that the modified technique was more sensitive in the detection of FB than the traditional method, with 16 out of 22 individuals showing positive identification against 14 out of 22, respectively. Furthermore, the modified technique proved not only to be more sensitive, but also almost twice as accurate: 49 FB/g vs. 26.7 FB/g of dry tissue.
史密斯和内勒的技术已被世界各地的许多作者成功用于从肺部回收无机颗粒。在这份简短的报告中，我们比较了通过两种不同方法从人肺组织中回收的无机颗粒计数结果：史密斯和内勒的技术及其一种改进方法。我们使用含铁小体（FB）作为无机颗粒的标志物，并比较了两种方法中FB计数的结果。在传统技术中，在1:1乙醇 - 氯仿混合物中形成的界面被排出，并且仅在形成的沉淀中对FB进行计数。我们的改进方法还对界面部分中的FB进行了定量。从22名个体的每个肺叶采集了尸检样本，共198个样本。每个样本用市售液体漂白剂消化，并通过两种技术进行处理。我们的结果表明，改进后的技术在检测FB方面比传统方法更敏感，22名个体中有16名通过改进技术检测出阳性，而传统方法为22名中的14名。此外，改进后的技术不仅更敏感，而且几乎精确两倍：每克干组织中分别为49个FB和26.7个FB。
