关于核糖体在“饥饿”密码子处移码的方向特异性。
On the directional specificity of ribosome frameshifting at a "hungry" codon.
作者信息
Lindsley D, Gallant J
机构信息
Department of Genetics, University of Washington, Seattle 98195.
出版信息
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5469-73. doi: 10.1073/pnas.90.12.5469.
Abstract
Limitation for aminoacyl-tRNA promotes ribosome frameshifting at certain sites. We have previously demonstrated ribosome frameshifting to the right (3') at an AAG site in one context, and to the left (5') at an AAG site in a different context. Here, we demonstrate that the "rightwing" context is largely specific for frameshifting to the right, and the "leftwing" context is largely specific for frameshifting to the left. Analysis of these context rules, and the conversion of a sequence that promotes leftward frameshifting to one that promotes rightward frameshifting, demonstrated here, permits us to define a minimal heptanucleotide sequence sufficient for shiftiness in each direction at an AAG codon whose lysyl-tRNA is in short supply.
摘要
氨酰-tRNA的限制会促进核糖体在某些位点发生移码。我们之前已经证明，在一种情况下核糖体在AAG位点向右（3'）移码，而在另一种不同情况下在AAG位点向左（5'）移码。在这里，我们证明“右翼”环境主要特异性地导致向右移码，而“左翼”环境主要特异性地导致向左移码。对这些环境规则的分析，以及在此展示的将促进向左移码的序列转换为促进向右移码的序列，使我们能够定义一个最小的七核苷酸序列，该序列足以使在赖氨酸-tRNA供应不足的AAG密码子处向每个方向的移码成为可能。
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