de las Marinas M D, Sanz M L, Diéguez I, Latasa M, Oehling A
Department of Allergology and Clinical Immunology, School of Medicine, University of Navarra, Pamplona, Spain.
J Investig Allergol Clin Immunol. 1993 Mar-Apr;3(2):80-5.
Many authors have described an increase in total serum IgE values during the first few months of immunotherapy (IT), followed by a subsequent decrease. In previous studies in patients undergoing medium-term IT, we detected an IgE synthesis suppressor factor (SF), which was modified during the course of IT. In this study, we investigated the influence of long-term IT on the production of SF and the lymphocyte response to its stimulus. Mixed bidirectional cultures of lymphocytes isolated from healthy individuals (n = 62) and allergic patients (n = 98) were carried out after different periods of IT. The resulting supernatants were subjected to affinity chromatography, from which two fractions were obtained: the first one, likely to contain SF, was added to lymphocyte cultures from 13 allergic patients undergoing no IT (LyG1) and 6 allergic patients with > 3 years of IT (LyG5), in order to assess its inhibitory effect on IgE synthesis. We found that patients undergoing IT for > 3 years presented a significant increase in SF production and an important lymphocyte response to exogenous SF from most of the groups studied, but little response to their own SF. These results could be ascribed to an alteration in the quantity and/or structure of the lymphocyte receptors likely to be sensitive to these factors induced by IT.
许多作者都描述过在免疫治疗（IT）的最初几个月里血清总IgE值会升高，随后下降。在之前对接受中期IT治疗的患者的研究中，我们检测到一种IgE合成抑制因子（SF），它在IT过程中会发生改变。在本研究中，我们调查了长期IT对SF产生以及淋巴细胞对其刺激的反应的影响。在不同时间段的IT治疗后，对从健康个体（n = 62）和过敏患者（n = 98）中分离出的淋巴细胞进行了双向混合培养。将所得上清液进行亲和层析，得到两个组分：第一个组分可能含有SF，将其添加到13名未接受IT治疗的过敏患者（LyG1）和6名接受IT治疗超过3年的过敏患者（LyG5）的淋巴细胞培养物中，以评估其对IgE合成的抑制作用。我们发现，接受IT治疗超过3年的患者SF产生显著增加，并且在所研究的大多数组中，淋巴细胞对外源SF有重要反应，但对自身SF的反应很小。这些结果可能归因于淋巴细胞受体的数量和/或结构发生改变，这些受体可能对IT诱导的这些因子敏感。
