水牛源金黄色葡萄球菌某些生化特性的研究。

Studies on some biochemical characteristics of Staphylococcus aureus of buffalo mammary origin.

作者信息

Varshney J P, Kapur M P, Sharma A

机构信息

Department of Veterinary Medicine, College of Veterinary Sciences, Haryana Agricultural University, India.

出版信息

Comp Immunol Microbiol Infect Dis. 1993 Oct;16(4):317-21. doi: 10.1016/0147-9571(93)90161-w.

DOI:10.1016/0147-9571(93)90161-w

PMID:8281745

Abstract

A total of 49 isolates of Staphylococcus aureus of buffalo mammary origin were studied for biochemical characteristics. Coagulase production, clumping factor, haemolytic activity, pigment production and fermentation of maltose and mannitol were employed to differentiate S. aureus from S. hyicus and S. intermedius. Out of 49 isolates, 97.95, 93.87, 93.87, 89.79, 95.91, 100.0, 95.91, 59.18, 100.0, 100.0, 100.0, 89.79, 91.83 and 100.0% isolates were positive for coagulase production, protein-A production, haemolysin production, thermostable nuclease production, deoxyribonuclease production, tellurite reduction, nitrate reduction, lipase production, phosphatase production, mannitol fermentation, glucose fermentation, M.R. test, V.P. test and pigment production respectively. The only isolate from which coagulase production could not be detected, however, showed haemolytic activity, protein-A productivity, pigmentation and mannitol fermentation. One of the protein-A negative isolate was coagulase positive and showed mannitol fermentation, pigmentation and haemolytic activity. The study revealed that the biochemical characteristics of S. aureus of buffalo mammary origin did not differ from those of cattle origin. Coagulase, haemolysin, thermostable nuclease, deoxyribonuclease, phosphatase, lipase, tellurite and nitrate reduction closely related with protein-A. The presence of protein-A seems to be as reliable an indicator for S. aureus of buffalo origin as is coagulase production.

摘要

对49株源自水牛乳腺的金黄色葡萄球菌分离株进行了生化特性研究。采用凝固酶产生、聚集因子、溶血活性、色素产生以及麦芽糖和甘露醇发酵情况来区分金黄色葡萄球菌与猪葡萄球菌和中间葡萄球菌。在49株分离株中，凝固酶产生、蛋白A产生、溶血素产生、耐热核酸酶产生、脱氧核糖核酸酶产生、亚碲酸盐还原、硝酸盐还原、脂肪酶产生、磷酸酶产生、甘露醇发酵、葡萄糖发酵、甲基红试验、V-P试验和色素产生的阳性分离株分别占97.95%、93.87%、93.87%、89.79%、95.91%、100.0%、95.91%、59.18%、100.0%、100.0%、100.0%、89.79%、91.83%和100.0%。然而，唯一一株未检测到凝固酶产生的分离株表现出溶血活性、蛋白A产生、色素沉着和甘露醇发酵。一株蛋白A阴性的分离株凝固酶呈阳性，并表现出甘露醇发酵、色素沉着和溶血活性。研究表明，源自水牛乳腺的金黄色葡萄球菌的生化特性与源自牛的金黄色葡萄球菌的生化特性没有差异。凝固酶、溶血素、耐热核酸酶、脱氧核糖核酸酶、磷酸酶、脂肪酶、亚碲酸盐和硝酸盐还原与蛋白A密切相关。对于源自水牛的金黄色葡萄球菌，蛋白A的存在似乎与凝固酶产生一样是可靠的指标。