Barbut F, Mario N, Delmée M, Gozian J, Petit J C
Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Paris, France.
FEMS Microbiol Lett. 1993 Dec 1;114(2):161-6. doi: 10.1111/j.1574-6968.1993.tb06567.x.
This study describes the use of a new and easy method called random amplified polymorphic DNA (RAPD) assay to distinguish strains of C. difficile. We used two single short primers (AP4 and AP5) with arbitrary nucleotide sequences in a polymerase chain reaction to amplify genomic DNA. The profiles observed after electrophoretic separation were able to distinguish 20 reference C. difficile strains previously serotyped by Delmée's method. The fingerprints of 11 epidemiologically unrelated C. difficile strains clearly yielded a DNA polymorphism between all the strains. Latterly, RAPD profiles of 11 C. difficile strains isolated from 2 independent suspected outbreaks showed, in each case, a predominant banding pattern corresponding to an epidemic strain. These results suggest that RAPD assay could be a valuable tool for epidemiological studies.
本研究描述了一种名为随机扩增多态性DNA（RAPD）分析的新型简便方法用于区分艰难梭菌菌株。我们在聚合酶链反应中使用了两个具有任意核苷酸序列的单链短引物（AP4和AP5）来扩增基因组DNA。电泳分离后观察到的图谱能够区分先前用德尔梅方法进行血清分型的20株艰难梭菌参考菌株。11株流行病学上不相关的艰难梭菌菌株的指纹图谱清楚地显示了所有菌株之间的DNA多态性。最近，从2起独立的疑似暴发中分离出的11株艰难梭菌菌株的RAPD图谱在每种情况下都显示出与流行菌株相对应的主要条带模式。这些结果表明，RAPD分析可能是流行病学研究的一种有价值的工具。
