Yura Y, Harada K, Tsujimoto H, Yoshida H, Sato M
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Oral Surg Oral Med Oral Pathol. 1993 Dec;76(6):723-8. doi: 10.1016/0030-4220(93)90042-3.
The effects of removal of the submandibular gland (sialoadenectomy) and administration of human urinary epidermal growth factor on the 9,10-dimethyl-1,2-benzanthracene-induced tumor formation were investigated with the use of a hamster cheek pouch model. Syrian hamsters were treated with 0.5% 9,10-dimethyl-1,2-benzanthracene for 6 weeks. Thereafter hamsters in group 1 underwent a sham operation and those in groups 2 and 3 underwent a sialoadenectomy. Subsequently, hamsters in groups 1 and 2 were given 0.9% sodium chloride and group 3 received the human urinary epidermal growth factor at a dose of 0.25 mg/kg body weight subcutaneously three times a week for 8 weeks. Sixteen weeks after the start of the experiment, the mean number of tumors that were less than 3-mm in diameter in groups 1 and 3 was significantly greater than that in group 2 (p < 0.05). The overall incidence and mean number of all carcinomas irrespective of size showed no differences among the experimental groups. These results indicate that epidermal growth factor synthesized in the submandibular gland may enhance the induction of cheek pouch tumor.
利用仓鼠颊囊模型，研究了切除下颌下腺（涎腺切除术）和给予人尿表皮生长因子对9,10 - 二甲基 - 1,2 - 苯并蒽诱导的肿瘤形成的影响。叙利亚仓鼠用0.5%的9,10 - 二甲基 - 1,2 - 苯并蒽处理6周。此后，第1组仓鼠接受假手术，第2组和第3组仓鼠接受涎腺切除术。随后，第1组和第2组仓鼠给予0.9%氯化钠，第3组仓鼠以0.25mg/kg体重的剂量皮下注射人尿表皮生长因子，每周3次，共8周。实验开始16周后，第1组和第3组直径小于3mm的肿瘤平均数量显著多于第2组（p<0.05）。无论大小，所有癌的总体发生率和平均数量在各实验组之间无差异。这些结果表明，下颌下腺合成的表皮生长因子可能增强颊囊肿瘤的诱导。
