Marconi P, Bistoni F, Pitzurra M
Boll Ist Sieroter Milan. 1976;55(6):471-4.
The Partigen Elution Test (P.E.T.), a partially quantitative method for the determination of Ig classes to which the antibodies against superficial Candida antigens belong, has been assessed. The sensitivity of the test is comparable to that of the Ouchterlony test. The P.E.T. has the advantage of simplicity, great reproducibility and absolute specificity. With P.E.T. the level of single class antibodies can be quantitated on the basis of the diameter of immunoprecipitation ring. It seems possible to establish a correlation between the number of precipitation lines in the Ouchterlony test and the diameter of the precipitation ring in LC-Partigen plates. In addition as association among the width of the ring diameter, the Ig classes implicated and the severity of Candida affection is apparent.
已对帕蒂根洗脱试验（P.E.T.）进行了评估，该试验是一种用于确定抗浅表念珠菌抗原抗体所属Ig类别的部分定量方法。该试验的灵敏度与双向免疫扩散试验相当。帕蒂根洗脱试验具有操作简单、重现性好和特异性强的优点。通过帕蒂根洗脱试验，可根据免疫沉淀环的直径对单一类别抗体的水平进行定量。似乎有可能在双向免疫扩散试验中沉淀线的数量与LC-帕蒂根平板中沉淀环的直径之间建立相关性。此外，环直径的宽度、所涉及的Ig类别与念珠菌感染的严重程度之间存在明显关联。
