Wolfisberg H, Schmutz A, Stotzer R, Thormann W
Department of Clinical Pharmacology, University of Berne, Switzerland.
J Chromatogr A. 1993 Oct 22;652(2):407-16. doi: 10.1016/0021-9673(93)83260-Y.
In an effort to evaluate the use of electrokinetic capillary technology for therapeutic and diagnostic drug monitoring, samples were analysed batchwise with an automated, high-throughput capillary electrophoretic instrument coupled to an inexpensive PC data acquisition and evaluation system. Examples studied included the capillary electrophoretic (HPCE) determination of bupivacaine in drain fluid collected after pulmonary surgery and the micellar electrokinetic capillary chromatographic (MECC) determination of antipyrine in human plasma. Analyses for antipyrine could be accomplished without any sample pretreatment whereas bupivacaine required extraction prior to analysis. Antipyrine determination was effected through external calibration using either peak areas, relative peak areas or peak heights. The intraday and interday reproducibilities (n = 15) of the evaluated concentrations were 1.5-3% and 5-6%, respectively. For bupivacaine, determination based on internal and external calibration employing peak areas and peak heights was investigated. The intraday and interday reproducibilities (n = 5) of bupivacaine concentrations were about 1% and 2%, respectively, for internal calibration and both about 5% for external calibration. The electrokinetic capillary data compared well with data obtained by gas chromatography (bupivacaine) and high-performance liquid chromatography (antipyrine).
为了评估电动毛细管技术在治疗药物监测和诊断药物监测中的应用，使用一台与廉价个人计算机数据采集和评估系统相连的自动化高通量毛细管电泳仪对样品进行分批分析。所研究的实例包括对肺手术后收集的引流液中布比卡因的毛细管电泳（HPCE）测定以及对人血浆中安替比林的胶束电动毛细管色谱（MECC）测定。安替比林的分析无需任何样品预处理，而布比卡因在分析前需要进行萃取。安替比林的测定通过使用峰面积、相对峰面积或峰高的外标法来完成。评估浓度的日内和日间重现性（n = 15）分别为1.5 - 3%和5 - 6%。对于布比卡因，研究了基于峰面积和峰高的内标法和外标法测定。布比卡因浓度的日内和日间重现性（n = 5），内标法分别约为1%和2%，外标法均约为5%。电动毛细管数据与气相色谱法（布比卡因）和高效液相色谱法（安替比林）获得的数据相比良好。
