Wang D, Jiang Z, Mu J, Li M, Wang B
Hua Xi Yi Ke Da Xue Xue Bao. 1993 Sep;24(3):250-3.
Two hybridomas designated as F7B7 and F7B9 secreting monoclonal anti-idiotypic antibodies against terminal trisaccharide of PGL-I were developed by fusion of SP2/0 cells and spleen cells of BALB/c mouse immunized with mouse monoclonal anti-trisaccharide of PGL-I (MAb1-E10F1). To characterize the F7B7, the following results were obtained. First, F7B7 reacted with MAb1-E10F1 specifically. Secondly, the cross ELISA neutralizing tests gave positive results. The binding of anti-trisaccharide positive serum with trisaccharide (contained in semi-synthetic antigen, NT-O-BSA) was inhibited F7B7 and the degree of inhibition showed dose-dependent manner. The binding of anti-trisaccharide positive serum with F7B7 was inhibited by NT-O-BSA and the degree of inhibition also showed dose-dependent manner. It was concluded that the hybridoma F7B7 is able to secrete monoclonal anti-idiotypic antibodies that bear the internal image of trisaccharide of PGL-I. The potentials and advantages of monoclonal anti-idiotypic antibody F7B7 as surrogate antigen in the serodiagnosis of leprosy have been discussed.
通过将SP2/0细胞与用小鼠抗PGL-I三糖单克隆抗体（MAb1-E10F1）免疫的BALB/c小鼠的脾细胞融合，获得了两种分泌针对PGL-I末端三糖的单克隆抗独特型抗体的杂交瘤，分别命名为F7B7和F7B9。为了鉴定F7B7，得到了以下结果。首先，F7B7与MAb1-E10F1特异性反应。其次，交叉ELISA中和试验呈阳性结果。F7B7抑制了抗三糖阳性血清与三糖（包含在半合成抗原NT-O-BSA中）的结合，且抑制程度呈剂量依赖性。NT-O-BSA抑制了抗三糖阳性血清与F7B7的结合，抑制程度也呈剂量依赖性。得出的结论是，杂交瘤F7B7能够分泌具有PGL-I三糖内影像的单克隆抗独特型抗体。讨论了单克隆抗独特型抗体F7B7作为替代抗原在麻风病血清诊断中的潜力和优势。
