Liu X, Wang D, Mu J
Hua Xi Yi Ke Da Xue Xue Bao. 1993 Sep;24(3):254-7.
Hybridoma (4C4) secreting monoclone anti-idiotype antibody (McAb2) against anti-phenolic glycolipid-I (PGL-I) antibody (Ab1) was produced by fusion of SP2/0 myeloma cells and spleen cells of syngeneic mice which had been previously tolerant to human IgM 4C4 monoclone anti-idiotype antibody was identified with a series of experiments including competitive and neutralizing inhibition ELISA. It was found that the binding of McAb2 with rabbit anti-PGL-I antibody could be competitively inhibited by NT-O-BSA (synthetic analog of PGL-I) and neutralized by polyclonal anti-PGL-I antibody derived from various origins (human or rabbit); McAb2 could block the binding of purified human Ab1 with NT-O-BSA. The assay of McAb2 as mimic antigen demonstrated that McAb2 could substitute for NT-O-BSA in leprosy serodiagnosis. These results show that anti-idiotype antibody produced by 4C4 is a monoclone anti-idiotype antibody bearing internal image of PGL-I and possibly can be used in leprosy serodiagnosis.
通过将SP2/0骨髓瘤细胞与同基因小鼠的脾细胞融合，制备出分泌针对抗酚糖脂-I（PGL-I）抗体（Ab1）的单克隆抗独特型抗体（McAb2）的杂交瘤（4C4）。该同基因小鼠先前已对人IgM 4C4单克隆抗独特型抗体产生耐受。通过包括竞争和中和抑制ELISA在内的一系列实验鉴定了4C4单克隆抗独特型抗体。结果发现，McAb2与兔抗PGL-I抗体的结合可被NT-O-BSA（PGL-I的合成类似物）竞争性抑制，并被源自不同来源（人或兔）的多克隆抗PGL-I抗体中和；McAb2可阻断纯化的人Ab1与NT-O-BSA的结合。将McAb2作为模拟抗原进行检测表明，McAb2可在麻风病血清学诊断中替代NT-O-BSA。这些结果表明，4C4产生的抗独特型抗体是具有PGL-I内影像的单克隆抗独特型抗体，可能可用于麻风病血清学诊断。
