Yasuda H, Hisanaga T, Maeda K, Kawabata T, Takigawa T, Terada M, Kikkawa R, Shigeta Y
Third Department of Medicine, Shiga University of Medical Science, Japan.
J Neurol Sci. 1993 Dec 1;120(1):64-70. doi: 10.1016/0022-510x(93)90026-u.
In order to produce a model of ischemic neuropathy with neural microvascular alterations, sodium laurate was injected into a femoral artery and saline into the contralateral artery at the mid-thigh level in Sprague-Dawley rats aged 11 weeks. In view of the dose-related findings, 0.3 mg sodium laurate dissolved in 0.1 ml saline was used for the main experiment. The laurate-injected leg showed paresis during the experimental period. On day 1-7, various stages of Wallerian degeneration and acute microvascular changes were found. At 1 month, regenerating myelinated nerve fibers (MNFs) were found at the central or total fascicular area mainly in the distal tibial nerve. Morphometric analyses suggested that MNFs other than regenerating fibers are atrophic ones. The proliferative changes of nutrient microvascular walls were striking. The percent of closed epineurial microvessels was significantly larger on the laurate-injected sides and inversely associated with the diameter of MNFs. At 7 months, the attenuation of MNFs was more prominent and microvascular alterations were persistently observed. These pathological findings resemble those of chronic ischemic neuropathy including diabetic neuropathy, suggesting that this neuropathy model might provide some valuable and useful clues for clarifying the pathogenesis of chronic ischemic neuropathy.
为了建立伴有神经微血管改变的缺血性神经病变模型，将月桂酸钠注入11周龄的Sprague-Dawley大鼠大腿中部水平的股动脉，对侧动脉注入生理盐水。鉴于剂量相关的研究结果，主要实验使用溶解于0.1 ml生理盐水中的0.3 mg月桂酸钠。在实验期间，注射月桂酸钠的腿出现轻瘫。在第1 - 7天，发现了华勒氏变性的各个阶段和急性微血管变化。1个月时，在主要位于胫神经远端的中央或整个束状区域发现了再生的有髓神经纤维（MNFs）。形态学分析表明，除了再生纤维外，MNFs是萎缩性的。营养性微血管壁的增殖性变化显著。注射月桂酸钠一侧的神经外膜微血管闭合百分比明显更高，且与MNFs直径呈负相关。7个月时，MNFs的衰减更为明显，且持续观察到微血管改变。这些病理结果与包括糖尿病性神经病变在内的慢性缺血性神经病变相似，表明该神经病变模型可能为阐明慢性缺血性神经病变的发病机制提供一些有价值且有用的线索。
