Zhang Z Q
Department of Public Health, School of Medicine, Yokohama City University, Kanagawa, Japan.
Microbiol Immunol. 1993;37(10):773-7. doi: 10.1111/j.1348-0421.1993.tb01704.x.
To determine whether the avidin-biotin complex enzyme-linked immunosorbent assay (ABC-E) is a potentially useful method for detection of herpes simplex virus type 1 (HSV-1) antibody in saliva, paired serum and saliva samples from 129 healthy individuals aged 18 to 25 years were collected simultaneously and subjected to a neutralization test (NT) for neutralizing antibody and also to an indirect ELISA (IE) and ABC-E for HSV-1 specific IgG detection. Compared with the results of NT, the sensitivities of the IE and ABC-E for serum were both 100% (45/45), and for saliva 82.2% (37/45) and 93.3% (42/45), respectively. The specificity of all these methods was 100% (84/84). With the same ABC-E method, a significant correlation (r = 0.66, P < 0.001) between the OD-difference (d-OD) values of positive serum and saliva samples was observed. Furthermore, the consistency of ABC-E for salivary antibody detection was confirmed with the paired serum and saliva samples which were collected from four individuals followed up for eight months. It was clear that the ABC-E method for saliva can be used in place of the NT and ABC-E method for serum for seroprevalence studying of HSV-1 infection.
为了确定抗生物素蛋白-生物素复合物酶联免疫吸附测定法（ABC-E）是否是检测唾液中单纯疱疹病毒1型（HSV-1）抗体的一种潜在有用方法，同时收集了129名年龄在18至25岁之间的健康个体的配对血清和唾液样本，并对其进行中和抗体的中和试验（NT），以及用于检测HSV-1特异性IgG的间接酶联免疫吸附测定法（IE）和ABC-E。与NT结果相比，IE和ABC-E检测血清的灵敏度均为100%（45/45），检测唾液的灵敏度分别为82.2%（37/45）和93.3%（42/45）。所有这些方法的特异性均为100%（84/84）。采用相同的ABC-E方法，观察到阳性血清和唾液样本的光密度差值（d-OD）之间存在显著相关性（r = 0.66，P < 0.001）。此外，通过对4名个体随访8个月收集的配对血清和唾液样本，证实了ABC-E检测唾液抗体的一致性。显然，用于唾液检测的ABC-E方法可替代用于血清检测的NT和ABC-E方法，用于HSV-1感染的血清流行率研究。
