Stavri D, Popescu C, Constantin S, Niculescu D, Stavri H, Fuiorea I, Nicolaescu M, Ambert L, Basacopol A
Cantacuzino Institute, Bucharest, Romania.
Arch Roum Pathol Exp Microbiol. 1990 Oct-Dec;49(4):331-8.
"Two-assay" tests (TAT), immunoenzymatic determination of both specific antibodies and mycobacterial antigens in sera of tuberculous and non-tuberculous subjects, was undertaken in our territorial conditions, where BCG vaccination is systematically applied and the prevalence of tuberculous infection is relatively high. The sensitivity of the method, calculated on 42 patients with active pulmonary tuberculosis and on 39 patients with post-tuberculosis syndromes is high, i.e. 0.952. The specificity of the method separately calculated for 44 young subjects (under 21 years old), for 78 healthy adults and for 201 lung diseased patients, bacteriologically not ascertained as tuberculosis at the moment of sera prelevation, varied between 0.830 and 0.489. "TAT", performed with crude immunologic reagents, produces false-positive reactions in early BCG vaccinated subjects. Method specificity low values in pulmonary non-tuberculous patients group may be partially explained by the difficulty in establishing the real relationships, in time, between host and mycobacteria, by the bacteriological method imperfections or sample prelevating methods. Our results certainly underestimate the diagnosis value of "TAT".
在我们所在地区开展了“双检测”试验（TAT），即采用免疫酶法测定结核病患者和非结核病患者血清中的特异性抗体和分枝杆菌抗原。该地区系统性地接种了卡介苗，结核感染率相对较高。以42例活动性肺结核患者和39例肺结核后综合征患者为对象计算得出，该方法的灵敏度较高，为0.952。分别以44名年轻受试者（21岁以下）、78名健康成年人和201名肺部疾病患者（在采集血清时经细菌学检查未确诊为结核病）为对象计算得出，该方法的特异性在0.830至0.489之间。使用粗制免疫试剂进行的“TAT”试验在早期接种卡介苗的受试者中会产生假阳性反应。肺部非结核患者组中该方法特异性的低值，部分原因可能是难以及时通过细菌学方法的缺陷或样本采集方法确定宿主与分枝杆菌之间的真实关系。我们的结果肯定低估了“TAT”的诊断价值。
