痘苗病毒编码的尿嘧啶DNA糖基化酶在病毒DNA复制中起关键作用。

The vaccinia virus-encoded uracil DNA glycosylase has an essential role in viral DNA replication.

作者信息

Millns A K, Carpenter M S, DeLange A M

机构信息

Department of Human Genetics, University of Manitoba, Winnipeg, Canada.

出版信息

Virology. 1994 Feb;198(2):504-13. doi: 10.1006/viro.1994.1061.

DOI:10.1006/viro.1994.1061

PMID:8291232

Abstract

The vaccinia virus conditional-lethal temperature-sensitive (ts) mutant ts4149 is, at the nonpermissive temperature, severely impaired in its ability to replicate its DNA genome. Compared to wild type, the amount of replication is suppressed by several orders of magnitude, and the little DNA that is replicated is not converted to mature linear genomes. We have demonstrated that this "DNA-" phenotype is not the result of a failure to produce early proteins. In agreement with the DNA- phenotype, intermediate and late gene expression were not detected. Marker rescue and DNA sequencing located the mutation in ts4149 to open reading frame D4. This gene has recently been shown to encode a 25-kDa protein with uracil DNA glycosylase activity (D. T. Stuart, C. Upton, M. A. Higman, E. G. Niles, and G. McFadden (1993), J. Virol. 67, 2503-2512). We speculate on the function of this "essential" viral repair enzyme and its role(s) in viral DNA replication.

摘要

痘苗病毒条件致死温度敏感（ts）突变体ts4149在非允许温度下，其DNA基因组复制能力严重受损。与野生型相比，复制量被抑制了几个数量级，并且少量复制的DNA未转化为成熟的线性基因组。我们已经证明这种“DNA-”表型不是未能产生早期蛋白的结果。与DNA-表型一致，未检测到中期和晚期基因表达。通过标记拯救和DNA测序将ts4149中的突变定位到开放阅读框D4。最近已证明该基因编码一种具有尿嘧啶DNA糖基化酶活性的25 kDa蛋白（D. T. Stuart、C. Upton、M. A. Higman、E. G. Niles和G. McFadden（1993年），《病毒学杂志》67，2503 - 2512）。我们推测这种“必需”病毒修复酶的功能及其在病毒DNA复制中的作用。