通过互补细胞系构建缺乏必需DNA修复酶尿嘧啶DNA糖基化酶的痘苗病毒。
Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line.
作者信息
Holzer G W, Falkner F G
机构信息
IMMUNO AG, Biomedical Research Center, Orth/Donau, Austria.
出版信息
J Virol. 1997 Jul;71(7):4997-5002. doi: 10.1128/JVI.71.7.4997-5002.1997.
Abstract
The vaccinia virus D4R open reading frame, encoding the essential DNA repair enzyme uracil DNA glycosylase, was expressed in two permanent cell lines, the rabbit kidney cell line RK13 and the human fibroblast cell line 293. The temperature-sensitive vaccinia virus mutant ts4149, which maps within D4R, was able to grow under restrictive conditions in both of these transformed cell lines. Cell clones complemented D4R function to various degrees, demonstrating complementation of an essential vaccinia virus gene by a cell line constitutively expressing the essential function. Thus, the complementing host cells allowed the rescue of a virus defective in the D4R gene, demonstrating that this system may be used for the propagation of defective cytoplasmic DNA viruses. The defective virus grew to high yields only in the engineered cell lines. The data support the hypothesis that early gene products, such as uracil DNA glycosylase, supplied in trans can fully complement essential viral functions.
摘要
编码必需的DNA修复酶尿嘧啶DNA糖基化酶的痘苗病毒D4R开放阅读框在两种永久细胞系中表达，即兔肾细胞系RK13和人成纤维细胞系293。定位在D4R内的温度敏感型痘苗病毒突变体ts4149能够在这两种转化细胞系的限制条件下生长。细胞克隆在不同程度上补充了D4R功能，证明了组成型表达必需功能的细胞系对必需痘苗病毒基因的互补作用。因此，互补宿主细胞使得D4R基因缺陷的病毒得以拯救，表明该系统可用于有缺陷的细胞质DNA病毒的增殖。有缺陷的病毒仅在工程细胞系中生长至高产量。数据支持这样的假设，即通过反式提供的早期基因产物，如尿嘧啶DNA糖基化酶，可以完全补充必需的病毒功能。
相似文献
1
Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line.通过互补细胞系构建缺乏必需DNA修复酶尿嘧啶DNA糖基化酶的痘苗病毒。
J Virol. 1997 Jul;71(7):4997-5002. doi: 10.1128/JVI.71.7.4997-5002.1997.
2
Mutations in active-site residues of the uracil-DNA glycosylase encoded by vaccinia virus are incompatible with virus viability.牛痘病毒编码的尿嘧啶-DNA糖基化酶活性位点残基的突变与病毒生存能力不兼容。
J Virol. 1996 Nov;70(11):7965-73. doi: 10.1128/JVI.70.11.7965-7973.1996.
3
Defective vaccinia virus as a biologically safe tool for the overproduction of recombinant human secretory proteins.缺陷痘苗病毒作为一种用于过量生产重组人分泌蛋白的生物安全工具。
Protein Expr Purif. 1998 Dec;14(3):317-26. doi: 10.1006/prep.1998.0970.
4
A poxvirus-encoded uracil DNA glycosylase is essential for virus viability.一种痘病毒编码的尿嘧啶DNA糖基化酶对病毒的生存能力至关重要。
J Virol. 1993 May;67(5):2503-12. doi: 10.1128/JVI.67.5.2503-2512.1993.
5
The vaccinia virus-encoded uracil DNA glycosylase has an essential role in viral DNA replication.痘苗病毒编码的尿嘧啶DNA糖基化酶在病毒DNA复制中起关键作用。
Virology. 1994 Feb;198(2):504-13. doi: 10.1006/viro.1994.1061.
6
Vaccinia virus uracil DNA glycosylase has an essential role in DNA synthesis that is independent of its glycosylase activity: catalytic site mutations reduce virulence but not virus replication in cultured cells.痘苗病毒尿嘧啶DNA糖基化酶在DNA合成中具有重要作用，该作用独立于其糖基化酶活性：催化位点突变会降低毒力，但不会影响病毒在培养细胞中的复制。
J Virol. 2003 Jan;77(1):159-66. doi: 10.1128/jvi.77.1.159-166.2003.
7
Isolation and characterization of a human cDNA encoding uracil-DNA glycosylase.编码尿嘧啶-DNA糖基化酶的人类cDNA的分离与鉴定。
Biochim Biophys Acta. 1991 Feb 16;1088(2):197-207. doi: 10.1016/0167-4781(91)90055-q.
8
Identification of human herpesvirus 6 uracil-DNA glycosylase gene.人类疱疹病毒6型尿嘧啶-DNA糖基化酶基因的鉴定
J Gen Virol. 1994 Sep;75 ( Pt 9):2349-54. doi: 10.1099/0022-1317-75-9-2349.
9
Identification of a poxvirus gene encoding a uracil DNA glycosylase.一种编码尿嘧啶DNA糖基化酶的痘病毒基因的鉴定。
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4518-22. doi: 10.1073/pnas.90.10.4518.
10
Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication.人巨细胞病毒尿嘧啶DNA糖基化酶对于DNA合成和病毒复制的正常时间调控是必需的。
J Virol. 1996 May;70(5):3018-25. doi: 10.1128/JVI.70.5.3018-3025.1996.
引用本文的文献
1
Correlated Target Search by Vaccinia Virus Uracil-DNA Glycosylase, a DNA Repair Enzyme and a Processivity Factor of Viral Replication Machinery.关联靶点搜索通过牛痘病毒尿嘧啶-DNA 糖基化酶，一种 DNA 修复酶和病毒复制机制的持续因子。
Int J Mol Sci. 2023 May 23;24(11):9113. doi: 10.3390/ijms24119113.
2
Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens.通过全基因组 CRISPR 遗传筛选鉴定 B2M 基因产物β2 微球蛋白为牛痘病毒感染的宿主因子。
PLoS Pathog. 2022 Dec 27;18(12):e1010800. doi: 10.1371/journal.ppat.1010800. eCollection 2022 Dec.
3
Targeting Nucleotide Biosynthesis: A Strategy for Improving the Oncolytic Potential of DNA Viruses.靶向核苷酸生物合成：一种提高DNA病毒溶瘤潜力的策略。
Front Oncol. 2017 Sep 26;7:229. doi: 10.3389/fonc.2017.00229. eCollection 2017.
4
Deletion of the Vaccinia Virus I2 Protein Interrupts Virion Morphogenesis, Leading to Retention of the Scaffold Protein and Mislocalization of Membrane-Associated Entry Proteins.痘苗病毒I2蛋白的缺失会中断病毒粒子的形态发生，导致支架蛋白的保留以及膜相关进入蛋白的定位错误。
J Virol. 2017 Jul 12;91(15). doi: 10.1128/JVI.00558-17. Print 2017 Aug 1.
5
Investigating Viruses during the Transformation of Molecular Biology.分子生物学变革时期的病毒研究
J Biol Chem. 2017 Mar 10;292(10):3958-3969. doi: 10.1074/jbc.X117.778712. Epub 2017 Jan 30.
6
Vaccinia Virus LC16m8∆ as a Vaccine Vector for Clinical Applications.痘苗病毒LC16m8∆作为临床应用的疫苗载体
Vaccines (Basel). 2014 Oct 17;2(4):755-71. doi: 10.3390/vaccines2040755.
7
Crystal Structure of the Vaccinia Virus Uracil-DNA Glycosylase in Complex with DNA.痘苗病毒尿嘧啶-DNA糖基化酶与DNA复合物的晶体结构
J Biol Chem. 2015 Jul 17;290(29):17923-17934. doi: 10.1074/jbc.M115.648352. Epub 2015 Jun 4.
8
A novel target and approach for identifying antivirals against molluscum contagiosum virus.一种用于鉴定抗传染性软疣病毒抗病毒药物的新靶点和方法。
Antimicrob Agents Chemother. 2014 Dec;58(12):7383-9. doi: 10.1128/AAC.03660-14. Epub 2014 Sep 29.
9
Crystal structure of the vaccinia virus DNA polymerase holoenzyme subunit D4 in complex with the A20 N-terminal domain.痘苗病毒DNA聚合酶全酶亚基D4与A20 N端结构域复合物的晶体结构
PLoS Pathog. 2014 Mar 6;10(3):e1003978. doi: 10.1371/journal.ppat.1003978. eCollection 2014 Mar.
10
Generation of a complete single-gene knockout bacterial artificial chromosome library of cowpox virus and identification of its essential genes.牛痘病毒完整单基因敲除细菌人工染色体文库的构建及其必需基因的鉴定。
J Virol. 2014 Jan;88(1):490-502. doi: 10.1128/JVI.02385-13. Epub 2013 Oct 23.
本文引用的文献
1
The purification fo four strains of poxvirus.四种痘病毒株的纯化
Virology. 1962 Sep;18:9-18. doi: 10.1016/0042-6822(62)90172-1.
2
Overexpression of human prothrombin in permanent cell lines using a dominant selection/amplification fusion marker.利用显性选择/扩增融合标记在永久细胞系中过表达人凝血酶原。
Protein Expr Purif. 1996 Nov;8(3):358-64. doi: 10.1006/prep.1996.0111.
3
Applications of pox virus vectors to vaccination: an update.痘病毒载体在疫苗接种中的应用：最新进展
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11349-53. doi: 10.1073/pnas.93.21.11349.
4
Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety.用于重组基因表达、疫苗接种和安全性的基因工程痘病毒。
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11341-8. doi: 10.1073/pnas.93.21.11341.
5
Evaluation of the thymidine kinase (tk) locus as an insertion site in the highly attenuated vaccinia MVA strain.评估胸苷激酶（tk）基因座作为高度减毒痘苗病毒MVA株插入位点的情况。
Arch Virol. 1996;141(3-4):663-9. doi: 10.1007/BF01718324.
6
A poxvirus-encoded uracil DNA glycosylase is essential for virus viability.一种痘病毒编码的尿嘧啶DNA糖基化酶对病毒的生存能力至关重要。
J Virol. 1993 May;67(5):2503-12. doi: 10.1128/JVI.67.5.2503-2512.1993.
7
Recent advances in retrovirus vector technology.逆转录病毒载体技术的最新进展。
Curr Opin Genet Dev. 1993 Feb;3(1):102-9. doi: 10.1016/s0959-437x(05)80349-1.
8
Identification of a poxvirus gene encoding a uracil DNA glycosylase.一种编码尿嘧啶DNA糖基化酶的痘病毒基因的鉴定。
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4518-22. doi: 10.1073/pnas.90.10.4518.
9
The vaccinia virus-encoded uracil DNA glycosylase has an essential role in viral DNA replication.痘苗病毒编码的尿嘧啶DNA糖基化酶在病毒DNA复制中起关键作用。
Virology. 1994 Feb;198(2):504-13. doi: 10.1006/viro.1994.1061.
10
Stable expression of the vaccinia virus K1L gene in rabbit cells complements the host range defect of a vaccinia virus mutant.痘苗病毒K1L基因在兔细胞中的稳定表达弥补了痘苗病毒突变体的宿主范围缺陷。
J Virol. 1994 Jul;68(7):4109-16. doi: 10.1128/JVI.68.7.4109-4116.1994.
Zotero 插件