[Microbiological methods of mineral water analysis].

The Spanish legislature has announced the required microbiological standards for bottled mineral waters. These requirements were the subject of "Decree 607/13th March 1975" and they are the following: -A total absence of parasites and pathogen microorganisms. -A total account of Fungus: absence in 100 ml. -A total account of coliforms: absence in 100 ml. -E. coli: entire absence in 100 ml. -A total account of Streptococcus, absence in 100 ml. -A total account of Clostridium sulphite reducing: absence in 100 ml. -A total absence of Pseudomonas aeruginosa in 100 ml. Several technical methods which have been adopted in Spain for the microbiological investigation of mineral waters have been described. For the first time the Spanish legislation has specified the total absence of Pseudomonas aeruginosa in mineral waters and techniques wich reveal the differentiation between Pseudomonas aeruginosa and other Pseudomonas by fluorescent pigments. The confirmation of diagnosis must be to carry out by the Director of National Reference Centre of Health.