Villand P, Olsen O A, Kleczkowski L A
Plant Molecular Biology Laboratory, Agricultural Research Council of Norway, As.
Plant Mol Biol. 1993 Dec;23(6):1279-84. doi: 10.1007/BF00042361.
PCR amplification of cDNA prepared from poly(A)+ RNA from aerial parts of Arabidopsis thaliana, using degenerate nucleotide primers based on conserved regions between the large and small subunits of ADP-glucose pyrophosphorylase (AGP), yielded four different cDNAs of ca. 550 nucleotides each. Based on derived amino acid sequences, the identities between the clones varied from 49 to 69%. Sequence comparison to previously published cDNAs for AGP from various species and tissues has revealed that three of the amplified cDNAs (ApL1, ApL2 and ApL3) correspond to the large subunit of AGP, and one cDNA (ApS) encodes the small subunit of AGP. Both ApL1 and ApS were subsequently found to be present in a cDNA library made from Arabidopsis leaves. All four PCR products are encoded by single genes, as found by genomic Southern analysis.
利用基于ADP - 葡萄糖焦磷酸化酶（AGP）大小亚基保守区域设计的简并核苷酸引物，对从拟南芥地上部分的聚腺苷酸加尾RNA（poly(A)+ RNA）制备的cDNA进行PCR扩增，得到了4种不同的cDNA，每种约550个核苷酸。根据推导的氨基酸序列，这些克隆之间的同一性在49%至69%之间。与先前发表的来自各种物种和组织的AGP的cDNA进行序列比较表明，扩增得到的3种cDNA（ApL1、ApL2和ApL3）对应于AGP的大亚基，一种cDNA（ApS）编码AGP的小亚基。随后发现ApL1和ApS都存在于从拟南芥叶片构建的cDNA文库中。如基因组Southern分析所示，所有4种PCR产物均由单基因编码。
