Akiba J, Ueno N, Chakrabarti B
Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Curr Eye Res. 1993 Oct;12(10):951-4. doi: 10.3109/02713689309020402.
To understand the molecular events underlying rheological vitreous changes, age-related changes in the molecular properties of bovine and human vitreous collagen were monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The adult bovine vitreous showed an increase in the high molecular-weight components of the collagen compared with the young samples. In addition, the apparent molecular size of the alpha-chain of the adult type II collagen was slightly larger than that of the young samples. The same tendency also was observed in human vitreous. The increase of high-molecular-weight components of the vitreous collagen could be attributed to intermolecular cross-links of the peptide chains.
为了解流变学玻璃体变化背后的分子事件，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳监测了牛和人玻璃体胶原蛋白分子特性的年龄相关变化。与年轻样本相比，成年牛玻璃体胶原蛋白的高分子量成分有所增加。此外，成年II型胶原蛋白α链的表观分子大小略大于年轻样本。在人玻璃体中也观察到了相同的趋势。玻璃体胶原蛋白高分子量成分的增加可归因于肽链的分子间交联。
