Pulido J S
Trans Am Ophthalmol Soc. 1996;94:1029-72.
To study the process of nonenzymatic glycosolation of vitreous collagen in vitro to determine the contributions of the classic Maillard pathway and the oxidative pathway, as well as to evaluate possible inhibitors of both pathways.
Bovine vitreous collagen was extracted and then incubated with hexoses in vitro. The amount of advanced glycosylation end (AGE) products was measured by fluorometry under varying conditions in the presence and absence of glycosolation inhibitors. Oxygen consumption studies and electron spin resonance spectroscopy with and without free-radical inhibitors were performed to differentiate oxidative from nonoxidative glycosolation.
Vitreous collagen undergoes nonenzymatic glycosolation in the presence of glucose or galactose in vitro. Oxygen consumption data show that oxygen is consumed in glucose and galactose solutions. Oxygen consumption is decreased by known free-radical inhibitors and rutin but not aminoguanidine. Electron spin resonance spectroscopy demonstrated the presence of a carbon-centered radical, and known free-radical inhibitors decreased the carbon-centered signal.
Nonenzymatic glycosolation of vitreous collagen can occur not only by the classic nonoxidative pathway, but also by a second oxidative pathway that is susceptible to a number of inhibitors.
研究玻璃体内胶原蛋白的非酶糖基化过程，以确定经典美拉德途径和氧化途径的作用，以及评估这两种途径可能的抑制剂。
提取牛玻璃体胶原蛋白，然后在体外与己糖孵育。在有和没有糖基化抑制剂的不同条件下，通过荧光法测量晚期糖基化终末（AGE）产物的量。进行有无自由基抑制剂的耗氧研究和电子自旋共振光谱分析，以区分氧化糖基化和非氧化糖基化。
玻璃体内胶原蛋白在体外葡萄糖或半乳糖存在的情况下会发生非酶糖基化。耗氧数据表明，葡萄糖和半乳糖溶液中会消耗氧气。已知的自由基抑制剂和芦丁可降低耗氧量，但氨基胍则无此作用。电子自旋共振光谱证明存在以碳为中心的自由基，已知的自由基抑制剂可降低以碳为中心的信号。
玻璃体内胶原蛋白的非酶糖基化不仅可通过经典的非氧化途径发生，还可通过第二种易受多种抑制剂影响的氧化途径发生。
