[从果蝇唾液腺分离细胞核制备多线染色体]
[Preparation of polytene chromosomes from isolated cell nuclei of the salivary glands of Drosophila].
作者信息
Smaragdov M G, Smirnov A F, Tolchkov E V
出版信息
Tsitologiia. 1976 Nov;18(11):1399-400.
PMID:829370
Abstract
A method is proposed for preparation of polytene chromosomes free of cytoplasmic substance. The nuclei are isolated using triton X-100 and squashed in 60% propionic acid solution.
摘要
提出了一种制备不含细胞质物质的多线染色体的方法。使用曲拉通X-100分离细胞核，并将其压片于60%的丙酸溶液中。
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