Evans R M, Baluda M A, Shoyab M
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3152-6. doi: 10.1073/pnas.71.8.3152.
The nature of integrated viral DNA in normal and leukemic chicken cells has been studied by sequential nucleic acid hybridization procedures that localize the viral specific DNA in cellular DNA regions differing in reiteration frequency. First, DNA.DNA reassociation was employed to fractionate cellular DNA sequences according to their reiteration frequencies. Next, the DNA in each fraction was denatured, immobilized on nitrocellulose filters, and then hybridized with viral [(3)H]RNA. In normal cells, endogenous viral DNA appears to be associated with cell sequences reiterated 1200 times, and each integration unit appears to have a maximal size approximately equivalent to the 35S RNA subunit of the virion. In infected cells, additional viral sequences are found which reassociate as if they integrated adjacent to unique cellular DNA, or in tandem with endogenous viral DNA.
通过连续核酸杂交程序研究了正常和白血病鸡细胞中整合病毒DNA的性质，该程序可将病毒特异性DNA定位在重复频率不同的细胞DNA区域。首先，利用DNA-DNA重退火根据重复频率对细胞DNA序列进行分级分离。接下来，将每个级分中的DNA变性，固定在硝酸纤维素滤膜上，然后与病毒[³H]RNA杂交。在正常细胞中，内源性病毒DNA似乎与重复1200次的细胞序列相关联，并且每个整合单元的最大大小似乎约等于病毒粒子的35S RNA亚基。在感染的细胞中，发现了额外的病毒序列，这些序列重退火时似乎整合在独特细胞DNA的相邻位置，或与内源性病毒DNA串联。
