Tomlinson A J, Benson L M, Johnson K L, Naylor S
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905.
J Chromatogr. 1993 Nov 24;621(2):239-48. doi: 10.1016/0378-4347(93)80101-9.
Combined capillary electrophoresis-mass spectrometry (CE-MS) is a powerful analytical tool for the characterization of components of complex mixtures. Furthermore, when interfaced with an electrospray ionization (ESI) source, skimmer induced fragmentation can yield structural information that aids compound identification. We show that these techniques can be used to probe the metabolic fate of drugs by investigation of the phase I metabolism of the widely used neuroleptic drug haloperidol. In addition, we demonstrate differences of in vitro metabolism of haloperidol by mouse and guinea pig hepatic microsomes.
毛细管电泳-质谱联用（CE-MS）是一种用于表征复杂混合物成分的强大分析工具。此外，当与电喷雾电离（ESI）源连接时，撇油器诱导的碎片化可以产生有助于化合物鉴定的结构信息。我们表明，通过研究广泛使用的抗精神病药物氟哌啶醇的I相代谢，这些技术可用于探究药物的代谢命运。此外，我们证明了小鼠和豚鼠肝微粒体对氟哌啶醇的体外代谢存在差异。
