Prabhasankar P, Ragupathi G, Sundaravadivel B, Annapoorani K S, Damodaran C
R & D Division, Forensic Sciences Department, Madras, India.
J Immunoassay. 1993 Dec;14(4):279-96. doi: 10.1080/15321819308019855.
An enzyme-linked immunosorbent assay is reported for monitoring thevetin, an active constituent of the highly poisonous plant Thevetia nerifolia. A thevetin-BSA conjugate was employed as the immunogen and the antibodies raised in rabbits were used for the development of an ELISA. Penicillinase served as the marker enzyme and its conjugation to thevetin by the periodate method is reported for the first time. The present ELISA method could detect 2 ng/ml of thevetin. Cross-reactivity studies with structural analogues and other phytotoxins and drugs of common occurrence in clinical and forensic toxicology established the superiority of the ELISA over the existing analytical methods for determining thevetin in various biospecimens.
据报道，一种酶联免疫吸附测定法用于监测黄夹苷，它是剧毒植物黄花夹竹桃的一种活性成分。使用黄夹苷-牛血清白蛋白缀合物作为免疫原，并将兔体内产生的抗体用于酶联免疫吸附测定法的开发。青霉素酶用作标记酶，首次报道了通过高碘酸盐法将其与黄夹苷偶联。目前的酶联免疫吸附测定法能够检测出2纳克/毫升的黄夹苷。与结构类似物以及临床和法医毒理学中常见的其他植物毒素和药物的交叉反应性研究证实，在测定各种生物样本中的黄夹苷时，该酶联免疫吸附测定法优于现有的分析方法。
