Zimmerman E R, Robertson K R, Kim H, Drobnis E Z, Nakajima S T
Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis 95616.
Fertil Steril. 1994 Feb;61(2):269-75.
To compare sperm migration through sodium hyaluronate with simple washing as methods for preparing sperm for IUI.
Ten normal semen specimens were prospectively collected and samples were prepared by simple washing and by migration into sodium hyaluronate using the Sperm Select System (Select Medical Systems, Williston, VT). The semen and each treatment group were evaluated for sperm concentration, percent motile, viability, acrosomal status, longevity, and computer-aided semen analysis (CASA) parameters.
University reproductive endocrinology facility.
The recovery of motile sperm was significantly higher for the washing method (mean +/- SEM 75% +/- 7%) than for the hyaluronate method (10% +/- 1%). The number of motile sperm recovered by migration into hyaluronate was independent of the percentage of motile sperm in the semen specimen and positively correlated with sperm concentration. The hyaluronate method produced greater percentages of motile, viable, and morphologically normal sperm, with lower proportions of premature acrosome reactions, higher sperm velocity, and greater linearity.
The Sperm Select System method of sperm separation provides a highly uniform specimen with improved sperm quality. However, the recovery of motile sperm is considerably lower than for simple washing methods.
比较经透明质酸钠处理的精子迁移与单纯洗涤作为宫腔内人工授精（IUI）精子制备方法的效果。
前瞻性收集10份正常精液标本，分别采用单纯洗涤法和使用精子筛选系统（Select Medical Systems，佛蒙特州威利斯顿）将精子迁移至透明质酸钠中的方法制备样本。对精液及各处理组的精子浓度、活动率、活力、顶体状态、存活时间和计算机辅助精液分析（CASA）参数进行评估。
大学生殖内分泌科。
洗涤法回收的活动精子显著高于透明质酸钠法（均值±标准误 75%±7% 对比 10%±1%）。迁移至透明质酸钠中回收的活动精子数量与精液标本中活动精子的百分比无关，而与精子浓度呈正相关。透明质酸钠法产生的活动、存活及形态正常的精子百分比更高，顶体反应过早的比例更低，精子速度更高，直线性更好。
精子筛选系统的精子分离方法可提供高度均匀的标本，精子质量有所改善。然而，活动精子的回收率远低于单纯洗涤法。
