Purification and characterisation of the restriction endonuclease ItaI from Ilyobacter tartaricus recognizing 5'-GC decreases NGC-3'.

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作者信息

Städtler P, von Strandmann R P, Walter T, Frey B, Auer H, Hengstenberg W, Schmitz G

机构信息

Department of Microbiology, Ruhr-Universität Bochum, Germany.

出版信息

Gene. 1993 Dec 31;137(2):347-8. doi: 10.1016/0378-1119(93)90033-y.

DOI:10.1016/0378-1119(93)90033-y

PMID:8299969

Abstract

ItaI, an isoschizomer of the subclass IIW [Kessler and Manta, Gene 92 (1990) 1-248] restriction endonuclease (ENase), Fnu4HI [Leung et al., Nucleic Acids Res. 6 (1979) 17-25], has been isolated from Ilyobacter tartaricus. The ENase has the five-base palindromic recognition sequence, 5'GC decreases NGC-3'. It cleaves behind the second nucleotide and produces a one-nt 5' overhang.

摘要