SgfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5'-GCGAT/CGC-3'.
作者信息
Kappelman J R, Brady M, Knoche K, Murray E, Schoenfeld T, Williams R, Vesselinova N
机构信息
Promega Corp., Madison, WI 53711-5399, USA.
出版信息
Gene. 1995 Jul 4;160(1):55-8. doi: 10.1016/0378-1119(95)00197-e.
PMID:7628716
Abstract
A new restriction endonuclease (ENase), SgfI, has been isolated from the bacterium Streptomyces sp. SgfI recognizes the 8-bp palindrome 5'-GCGATCGC-3' and cleaves double-stranded DNA after the T in this sequence, producing a two-base 3' overhang compatible with PvuI termini. SgfI is a rare-cutting ENase and should be useful for megabase mapping experiments.
摘要
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