• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过随机诱变鉴定出肺炎溶血素中对溶血活性重要的氨基酸。

Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis.

作者信息

Hill J, Andrew P W, Mitchell T J

机构信息

Department of Microbiology, University of Leicester, United Kingdom.

出版信息

Infect Immun. 1994 Feb;62(2):757-8. doi: 10.1128/iai.62.2.757-758.1994.

DOI:10.1128/iai.62.2.757-758.1994
PMID:8300240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC186175/
Abstract

The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

摘要

肺炎溶血素的结构基因被随机诱变。开发了一种筛选方法来鉴定溶血活性降低的突变体，该方法鉴定出含半胱氨酸区域内以及毒素N端残基处的替代。这些是首次报道的毒素N端区域内影响细胞溶解活性的变化。

相似文献

1
Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis.通过随机诱变鉴定出肺炎溶血素中对溶血活性重要的氨基酸。
Infect Immun. 1994 Feb;62(2):757-8. doi: 10.1128/iai.62.2.757-758.1994.
2
Comparative virulence of Streptococcus pneumoniae strains with insertion-duplication, point, and deletion mutations in the pneumolysin gene.肺炎链球菌菌株在肺炎溶血素基因中存在插入重复、点突变和缺失突变时的相对毒力
Infect Immun. 1999 Feb;67(2):981-5. doi: 10.1128/IAI.67.2.981-985.1999.
3
Sequence variation in the Streptococcus pneumoniae pneumolysin gene affecting haemolytic activity and electrophoretic mobility of the toxin.肺炎链球菌溶血素基因中的序列变异影响毒素的溶血活性和电泳迁移率。
Microb Pathog. 1996 Aug;21(2):71-83. doi: 10.1006/mpat.1996.0044.
4
Effect of defined point mutations in the pneumolysin gene on the virulence of Streptococcus pneumoniae.肺炎溶血素基因特定点突变对肺炎链球菌毒力的影响。
Infect Immun. 1995 May;63(5):1969-74. doi: 10.1128/iai.63.5.1969-1974.1995.
5
Amino acid changes affecting the activity of pneumolysin alter the behaviour of pneumococci in pneumonia.影响肺炎球菌溶血素活性的氨基酸变化会改变肺炎链球菌在肺炎中的行为。
Microb Pathog. 1998 Mar;24(3):167-74. doi: 10.1006/mpat.1997.0185.
6
Molecular cloning, characterization, and complete nucleotide sequence of the gene for pneumolysin, the sulfhydryl-activated toxin of Streptococcus pneumoniae.肺炎链球菌巯基激活毒素——肺炎溶血素基因的分子克隆、特性鉴定及全核苷酸序列分析
Infect Immun. 1987 May;55(5):1184-9. doi: 10.1128/iai.55.5.1184-1189.1987.
7
The role of pneumolysin in ocular infections with Streptococcus pneumoniae.肺炎溶血素在肺炎链球菌眼部感染中的作用。
Curr Eye Res. 1990 Nov;9(11):1107-14. doi: 10.3109/02713689008997584.
8
Pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae, does not require a thiol group for in vitro activity.肺炎溶素是肺炎链球菌的硫醇激活毒素，其体外活性并不需要硫醇基团。
Infect Immun. 1989 Aug;57(8):2547-52. doi: 10.1128/iai.57.8.2547-2552.1989.
9
Reduced virulence of a defined pneumolysin-negative mutant of Streptococcus pneumoniae.肺炎链球菌特定肺炎溶血素阴性突变体的毒力降低
Infect Immun. 1989 Jul;57(7):2037-42. doi: 10.1128/iai.57.7.2037-2042.1989.
10
Effect of insertional inactivation of the genes encoding pneumolysin and autolysin on the virulence of Streptococcus pneumoniae type 3.编码肺炎溶素和自溶素的基因插入失活对3型肺炎链球菌毒力的影响。
Microb Pathog. 1992 Feb;12(2):87-93. doi: 10.1016/0882-4010(92)90111-z.

引用本文的文献

1
The Yin and Yang of Pneumolysin During Pneumococcal Infection.肺炎链球菌感染中的肺炎球菌溶血素的阴阳两面。
Front Immunol. 2022 Apr 22;13:878244. doi: 10.3389/fimmu.2022.878244. eCollection 2022.
2
Single nucleotide variations: biological impact and theoretical interpretation.单核苷酸变异：生物学影响与理论阐释
Protein Sci. 2014 Dec;23(12):1650-66. doi: 10.1002/pro.2552. Epub 2014 Oct 20.
3
THP-1 monocytes up-regulate intercellular adhesion molecule 1 in response to pneumolysin from Streptococcus pneumoniae.THP-1单核细胞对肺炎链球菌的肺炎溶血素产生反应，上调细胞间黏附分子1。
Infect Immun. 2005 Oct;73(10):6493-8. doi: 10.1128/IAI.73.10.6493-6498.2005.
4
The role of pneumolysin in pneumococcal pneumonia and meningitis.肺炎溶血素在肺炎球菌肺炎和脑膜炎中的作用。
Clin Exp Immunol. 2004 Nov;138(2):195-201. doi: 10.1111/j.1365-2249.2004.02611.x.
5
Pneumococcal virulence factors: structure and function.肺炎球菌毒力因子：结构与功能
Microbiol Mol Biol Rev. 2001 Jun;65(2):187-207 ; first page, table of contents. doi: 10.1128/MMBR.65.2.187-207.2001.

本文引用的文献

1
Properties of purified pneumococcal hemolysin.纯化肺炎球菌溶血素的特性
Infect Immun. 1972 Nov;6(5):755-60. doi: 10.1128/iai.6.5.755-760.1972.
2
Nucleotide sequence of the streptolysin O (SLO) gene: structural homologies between SLO and other membrane-damaging, thiol-activated toxins.链球菌溶血素O（SLO）基因的核苷酸序列：SLO与其他膜损伤性硫醇激活毒素之间的结构同源性
Infect Immun. 1987 Dec;55(12):3228-32. doi: 10.1128/iai.55.12.3228-3232.1987.
3
A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro.一种全新的完整基因随机诱变方法：体外酶促生成突变文库。
Protein Eng. 1988 Apr;2(1):63-8. doi: 10.1093/protein/2.1.63.
4
Identification of the structural gene encoding the SH-activated hemolysin of Listeria monocytogenes: listeriolysin O is homologous to streptolysin O and pneumolysin.编码单核细胞增生李斯特菌SH激活溶血素的结构基因的鉴定：李斯特菌溶血素O与链球菌溶血素O及肺炎溶血素同源。
Infect Immun. 1987 Dec;55(12):3225-7. doi: 10.1128/iai.55.12.3225-3227.1987.
5
Pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae, does not require a thiol group for in vitro activity.肺炎溶素是肺炎链球菌的硫醇激活毒素，其体外活性并不需要硫醇基团。
Infect Immun. 1989 Aug;57(8):2547-52. doi: 10.1128/iai.57.8.2547-2552.1989.
6
Reduced virulence of a defined pneumolysin-negative mutant of Streptococcus pneumoniae.肺炎链球菌特定肺炎溶血素阴性突变体的毒力降低
Infect Immun. 1989 Jul;57(7):2037-42. doi: 10.1128/iai.57.7.2037-2042.1989.
7
Expression of the pneumolysin gene in Escherichia coli: rapid purification and biological properties.
Biochim Biophys Acta. 1989 Jan 23;1007(1):67-72. doi: 10.1016/0167-4781(89)90131-0.
8
Site-directed mutagenesis of the hole-forming toxin aerolysin: studies on the roles of histidines in receptor binding and oligomerization of the monomer.
Biochemistry. 1990 Feb 27;29(8):2177-80. doi: 10.1021/bi00460a031.
9
Attenuated mutants of the intracellular bacterium Listeria monocytogenes obtained by single amino acid substitutions in listeriolysin O.通过对溶血素O进行单个氨基酸替换获得的胞内细菌单核细胞增生李斯特菌的减毒突变体。
Mol Microbiol. 1990 Dec;4(12):2167-78. doi: 10.1111/j.1365-2958.1990.tb00578.x.
10
CLUSTAL V: improved software for multiple sequence alignment.CLUSTAL V：用于多序列比对的改进软件。
Comput Appl Biosci. 1992 Apr;8(2):189-91. doi: 10.1093/bioinformatics/8.2.189.