通过随机诱变鉴定出肺炎溶血素中对溶血活性重要的氨基酸。
Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis.
作者信息
Hill J, Andrew P W, Mitchell T J
机构信息
Department of Microbiology, University of Leicester, United Kingdom.
出版信息
Infect Immun. 1994 Feb;62(2):757-8. doi: 10.1128/iai.62.2.757-758.1994.
Abstract
The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.
摘要
肺炎溶血素的结构基因被随机诱变。开发了一种筛选方法来鉴定溶血活性降低的突变体，该方法鉴定出含半胱氨酸区域内以及毒素N端残基处的替代。这些是首次报道的毒素N端区域内影响细胞溶解活性的变化。
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