Willshaw G A, Smith H R, Roberts D, Thirlwell J, Cheasty T, Rowe B
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
J Appl Bacteriol. 1993 Nov;75(5):420-6. doi: 10.1111/j.1365-2672.1993.tb02797.x.
Fifty-four of 310 (17%) samples of raw beef products contained Vero cytotoxin (VT)-producing Escherichia coli (VTEC) detected by DNA probes for the VT genes. VTEC strains examined in detail from a selection of the positive samples belonged to several O serogroups, some of which have been associated with human diarrhoea or haemolytic uraemic syndrome. Some of the strains possessed properties that may contribute to virulence in man. None of the food samples contained VT-producing E. coli O157 when tested by a combination of VT probe tests and colony immunoblotting with commercially available anti-O157 serum. Quantification of the immunoblotting technique indicated that O157 VTEC could be recovered from artificially-inoculated meat samples at a level of less than one organism per gram. Five of the food samples carried E. coli O157 strains that did not produce VT and differed in other properties from O157 VTEC.
通过针对VT基因的DNA探针检测，310份生牛肉制品样本中有54份（17%）含有产Vero细胞毒素（VT）的大肠杆菌（VTEC）。从部分阳性样本中详细检测的VTEC菌株属于几个O血清群，其中一些与人类腹泻或溶血尿毒综合征有关。一些菌株具有可能导致人类致病的特性。当通过VT探针检测和用市售抗O157血清进行菌落免疫印迹相结合的方法检测时，没有一份食品样本含有产VT的大肠杆菌O157。免疫印迹技术的定量分析表明，从人工接种的肉类样本中可以检出O157 VTEC，其水平低于每克一个菌。5份食品样本携带不产VT的大肠杆菌O157菌株，这些菌株在其他特性上与O157 VTEC不同。
