Ubbink J B, Lagendijk J, Vermaak W H
Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, South Africa.
J Chromatogr. 1993 Oct 29;620(2):254-9. doi: 10.1016/0378-4347(93)80013-t.
An isocratic HPLC method is described to determine urinary concentrations of nicotine and cotinine after derivatization with cyanogen chloride and barbituric acid. This method has been used to assess the reliability of the direct barbituric acid assay to determine smoking status. It is concluded that the direct barbituric acid assay is a very reliable indicator of smoking status, provided that urine blank samples are prepared to correct for background absorbance. If the direct barbituric acid assay is in disagreement with self-reported smoking status, this HPLC procedure is a useful method to resolve the discrepancy.
本文描述了一种等度高效液相色谱法，用于测定用氯化氰和巴比妥酸衍生化后尿液中尼古丁和可替宁的浓度。该方法已用于评估直接巴比妥酸测定法在确定吸烟状况方面的可靠性。得出的结论是，只要制备尿液空白样品以校正背景吸光度，直接巴比妥酸测定法就是吸烟状况的非常可靠的指标。如果直接巴比妥酸测定法与自我报告的吸烟状况不一致，这种高效液相色谱法是解决差异的有用方法。
