Barlow R D, Stone R B, Wald N J, Puhakainen E V
Clin Chim Acta. 1987 May 29;165(1):45-52. doi: 10.1016/0009-8981(87)90217-8.
The qualitative direct barbituric acid (DBA) method of detecting urine nicotine metabolites was modified to make it quantitative. The performance of the quantitative DBA method was compared with the qualitative method and an established cotinine radio-immunoassay (RIA), using a panel of urines from 128 reported smokers and 383 reported non-smokers. The quantitative DBA method results were highly correlated with the cotinine RIA results, r = 0.85. The coefficients of variation for the two methods were 6% and 10%, respectively. Assuming that the reported smoking history was correct the qualitative DBA method gave a smoking detection rate of 91% and a false positive rate of 3%. At cut-off levels chosen to yield the same false positive rate the quantitative DBA method detected 93% of smokers, close to that of 98% detected with the cotinine RIA. The quantitative DBA method can be used to analyse over 170 samples per day compared to about 70 per day by RIA. It is therefore a fast and inexpensive alternative to cotinine assays for the assessment of smoking status and cigarette smoke intake.
对检测尿液中尼古丁代谢物的定性直接巴比妥酸（DBA）方法进行了改进，使其能够定量。使用来自128名报告吸烟者和383名报告非吸烟者的一组尿液，将定量DBA方法的性能与定性方法以及既定的可替宁放射免疫测定法（RIA）进行了比较。定量DBA方法的结果与可替宁RIA结果高度相关，r = 0.85。两种方法的变异系数分别为6%和10%。假设报告的吸烟史正确，定性DBA方法的吸烟检测率为91%，假阳性率为3%。在选择产生相同假阳性率的临界值时，定量DBA方法检测出93%的吸烟者，接近可替宁RIA检测出的98%。定量DBA方法每天可分析170多个样本，而RIA每天约为70个。因此，对于评估吸烟状况和香烟烟雾摄入量，它是一种快速且廉价的可替代可替宁检测的方法。
