Puhakainen E V, Barlow R D, Salonen J T
Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.
Clin Chim Acta. 1987 Dec;170(2-3):255-62. doi: 10.1016/0009-8981(87)90135-5.
We have modified the direct barbituric acid (DBA) test for urine nicotine metabolites so that it can be used on a continuous flow auto-analyser. The performance of the method was compared with a cotinine radio-immunoassay (RIA). The auto-analyser DBA method and cotinine RIA performed equally well in terms of assessing smoking status, yielding a false positive rate of 1.5% at a detection rate of 98% when compared to self-reported smoking information. The results obtained with the two methods were correlated, r = 0.91, although the DBA method results were consistently higher than the corresponding cotinine RIA results; the method detects cotinine and other nicotine metabolites. The coefficient of variation for the DBA method was 3.4% compared with 10% for the RIA. The DBA method has a throughput of about 250 samples per day compared to about 70 per day by RIA and the reagents are readily obtained and inexpensive.
我们对尿液尼古丁代谢物的直接巴比妥酸（DBA）检测方法进行了改进，使其能够用于连续流动自动分析仪。将该方法的性能与可替宁放射免疫分析（RIA）进行了比较。在评估吸烟状况方面，自动分析仪DBA方法和可替宁RIA表现相当，与自我报告的吸烟信息相比，在检测率为98%时，假阳性率为1.5%。两种方法获得的结果具有相关性，r = 0.91，尽管DBA方法的结果始终高于相应的可替宁RIA结果；该方法可检测可替宁和其他尼古丁代谢物。DBA方法的变异系数为3.4%，而RIA为10%。DBA方法的通量约为每天250个样本，相比之下，RIA约为每天70个样本，并且该方法的试剂容易获得且价格便宜。
