简单直接进样高效液相色谱法测定血浆中的奎尼丁。

Simple direct injection high-performance liquid chromatographic method to determine quinidine in plasma.

作者信息

Huang J L, Morgan D J

机构信息

Victorian College of Pharmacy, Monash University, Parkville, Melbourne, Australia.

出版信息

J Chromatogr. 1993 Oct 29;620(2):278-80. doi: 10.1016/0378-4347(93)80017-x.

DOI:10.1016/0378-4347(93)80017-x

PMID:8300799

Abstract

High-performance liquid chromatographic methods that use direct injection of plasma include column-switching procedures, modified mobile phases and small-pore modified stationary phases. By using a large-pore (300 A) Selectosil C18 column, developed for the analysis of macromolecules, we have shown that quinidine in plasma and protein solutions can be assayed accurately and rapidly by directly injecting 2 microliters plasma or protein solution onto the column. Column life is not reduced, and the limit of quantitation is 0.01 microM.

摘要

采用直接进样血浆的高效液相色谱方法包括柱切换程序、改良流动相和小孔径改性固定相。通过使用为分析大分子而开发的大孔径（300 Å）Selectosil C18柱，我们已经证明，直接将2微升血浆或蛋白质溶液注入该柱，就可以准确、快速地测定血浆和蛋白质溶液中的奎尼丁。柱寿命不会降低，定量限为0.01微摩尔。