Andrade-Mena C E, Sardo-Olmedo J A, Ramirez-Lizardo E J
Depto. de Invest. Cientifica, Facultad de Medicina, Universidad de Guadalajara, Mexico.
J Neuroimmunol. 1994 Feb;50(1):3-7. doi: 10.1016/0165-5728(94)90208-9.
The results obtained in these series of experiments indicate that oral administration of phenytoin (100, 50, or 25 mg/kg) to mice significantly depressed both humoral and cellular immune responses, evaluated by the techniques of enumeration of direct and indirect spleen plaque-forming cells (PFC) and the delayed-type hypersensitivity reaction (DTH) against sheep red blood cells (SR BC), when compared with those observed in normal control animals. Furthermore, spleen cells, purified splenic T lymphocytes or Ly 2 + T cells obtained from 100 mg/kg phenytoin-treated donor mice were capable of diminishing both PFC and DTH responses of normal cells transferred into lethally irradiated mice. The immunodepressor effect of phenytoin was observed despite the fact that administration of this drug induced a rise in spleen cellularity.
在这些系列实验中获得的结果表明，与正常对照动物相比，给小鼠口服苯妥英（100、50或25mg/kg）通过直接和间接脾空斑形成细胞（PFC）计数技术以及针对绵羊红细胞（SR BC）的迟发型超敏反应（DTH）评估，显著抑制了体液免疫和细胞免疫反应。此外，从接受100mg/kg苯妥英处理的供体小鼠获得的脾细胞、纯化的脾T淋巴细胞或Ly 2 + T细胞能够减弱转移到致死性照射小鼠体内的正常细胞的PFC和DTH反应。尽管给予该药物会导致脾细胞增多，但仍观察到苯妥英的免疫抑制作用。
