Yasuda T, Momma N, Ohsawa K
Tohoku College of Pharmacy, Miyagi, Japan.
Yakugaku Zasshi. 1993 Dec;113(12):881-9. doi: 10.1248/yakushi1947.113.12_881.
A simple and precise method was established for the simultaneous determination of daidzin and puerarin and the determination of daidzein in oriental pharmaceutical decoctions containing Puerariae Radix using high-performance liquid chromatography with tetra-n-heptylammonium bromide (THA) as an ion-pair reagent. Daidzin and puerarin were eluted within 45 min without interference with co-existing components using an ODS column and a mixture of 10 mM phosphate buffer (pH 6.5)-methanol (68:32) containing 5 mM THA as a mobile phase. Daidzein was eluted within 35 min without interference with co-existing components using an ODS column and a mixture of 10 mM phosphate buffer (pH 6.5)-acetonitrile (72:28 or 68:32) containing 5 mM THA as a mobile phase.
建立了一种简单精确的方法，采用以溴化四正庚基铵（THA）为离子对试剂的高效液相色谱法，同时测定含葛根的东方药汤中大豆苷和葛根素，并测定大豆苷元。使用ODS柱，以含5 mM THA的10 mM磷酸盐缓冲液（pH 6.5）-甲醇（68:32）混合物为流动相，大豆苷和葛根素在45分钟内洗脱，不受共存成分干扰。使用ODS柱，以含5 mM THA的10 mM磷酸盐缓冲液（pH 6.5）-乙腈（72:28或68:32）混合物为流动相，大豆苷元在35分钟内洗脱，不受共存成分干扰。
