[离子对高效液相色谱法同时测定含吴茱萸的中药汤剂中吴茱萸碱和吴茱萸次碱的含量]
[A simultaneous determination of evodiamine and rutaecarpine in oriental pharmaceutical decoctions containing Evodia fruit by ion-pair high-performance liquid chromatography].
作者信息
Ueda J, Momma N, Yamauchi Y, Yoshie F, Ohsawa K
机构信息
Tohoku College of Pharmacy, Sendai, Miyagi, Japan.
出版信息
Yakugaku Zasshi. 1996 Jan;116(1):59-64. doi: 10.1248/yakushi1947.116.1_59.
A simple and precise method was established for the simultaneous determination of evodiamine and rutaecarpine in oriental pharmaceutical decoctions containing Evodia Fruit using high-performance liquid chromatography with sodium dodecyl sulfate (SDS) as an ion-pair reagent. Evodiamine and rutaecarpine were eluted within 60 min without interference from co-existing components using an ODS column and a mixture of water-methanol-acetonitrile-SDS-phosphoric acid (600:330:70:5:0.01, v/v/v/w/v, pH 5.0) as a mobile phase.
建立了一种简单而精确的方法，以十二烷基硫酸钠（SDS）作为离子对试剂，采用高效液相色谱法同时测定含吴茱萸的中药汤剂中吴茱萸碱和吴茱萸次碱的含量。使用ODS柱，以水-甲醇-乙腈-SDS-磷酸（600:330:70:5:0.01，v/v/v/w/v，pH 5.0）的混合物作为流动相，吴茱萸碱和吴茱萸次碱在60分钟内洗脱完毕，且不受共存成分的干扰。
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