Güvenç Melih Güven, Midilli Kenan, Ozdoğan Ahmet, Inci Ender, Tahamiler Rauf, Enver Ozgün, Sirin Gözde, Ergin Sevgi, Kuşkucu Mert, Divanoğlu Emel Ozergil, Yilmaz Gülden, Altas Kemal
Istanbul University, Cerrahpaşa Medical Faculty, Department of Otorhinolaryngology, Turkey.
Auris Nasus Larynx. 2008 Sep;35(3):357-62. doi: 10.1016/j.anl.2007.08.006. Epub 2007 Oct 30.
In this study we aimed to investigate the presence of human herpesvirus 8 (HHV-8) and human papillomavirus (HPV) in laryngeal carcinoma.
Fifty patients operated on because of laryngeal carcinoma were included in the study. Forty-seven had squamous cell carcinoma (SCC) whereas three had verrucous carcinoma. Fresh tumoral tissues, or tumoral tissues obtained from archival paraffin-embedded blocks, were examined. HHV-8 DNA and HPV DNA were detected using polymerase chain reaction (PCR) and viral genotypes of HPV were determined via the hybrid capture method. The presence of HHV-8 DNA and HPV DNA were also investigated in normal appearing laryngeal tissue collected from 50 cadavers at autopsy.
HPV DNA was detected in seven patients (7/50; 14%) (5 out of 47 patients with SCC (5/47; 10.6%) and two out of three patients with verrucous carcinoma). HHV-8 DNA was detected in five patients and they all had SCC (5/47; 10.6%). One case had both HHV-8 and HPV DNA. None of the control samples from cadavers harbored HHV-8 DNA, or HPV DNA. There was a statistically significant correlation between HHV-8 DNA and HPV DNA positivity and laryngeal SCC (Fisher exact test; p=0.023 for each). No statistically significant correlations were found between the presence of HHV-8 and/or HPV and age, gender, tumor stage, differentiation, the site of the tumor, smoking and alcohol use.
The findings of the present study suggest that beside HPV, HHV-8 might have a role in laryngeal carcinogenesis. Further investigations are necessary to clarify the exact role of these viruses in laryngeal carcinoma.
在本研究中，我们旨在调查喉癌中人类疱疹病毒8型（HHV-8）和人乳头瘤病毒（HPV）的存在情况。
本研究纳入了50例因喉癌接受手术的患者。其中47例为鳞状细胞癌（SCC），3例为疣状癌。对新鲜肿瘤组织或从存档石蜡包埋块中获取的肿瘤组织进行检查。使用聚合酶链反应（PCR）检测HHV-8 DNA和HPV DNA，并通过杂交捕获法确定HPV的病毒基因型。还对50例尸体解剖时收集的外观正常的喉组织进行HHV-8 DNA和HPV DNA检测。
7例患者（7/50；14%）检测到HPV DNA（47例SCC患者中有5例（5/47；10.6%），3例疣状癌患者中有2例）。5例患者检测到HHV-8 DNA，且均为SCC（5/47；10.6%）。1例患者同时检测到HHV-8和HPV DNA。尸体对照样本中均未检测到HHV-8 DNA或HPV DNA。HHV-8 DNA和HPV DNA阳性与喉SCC之间存在统计学显著相关性（Fisher精确检验；两者p均=0.023）。未发现HHV-8和/或HPV的存在与年龄、性别、肿瘤分期、分化程度、肿瘤部位、吸烟和饮酒之间存在统计学显著相关性。
本研究结果表明，除HPV外，HHV-8可能在喉癌发生中起作用。需要进一步研究以阐明这些病毒在喉癌中的确切作用。
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