Keil L C, Severs W B
Endocrinology. 1977 Jan;100(1):30-8. doi: 10.1210/endo-100-1-30.
The development of a sensitive radioimmunoassay for plasma arginine vasopressin (pAVP) is described. Using this assay, the levels of vasopressin were determined in the plasma of nondehydrated and dehydrated rats after exposure to ether or acceleration stress. Plasma AVP was also determined in rats following nicotine administration. Nondehydrated rats showed no significant changes in pAVP 1, 2, 5, or 15 min after exposure to ether for 1 min. Dehydrated rats, on the other hand, had significantly reduced pAVP after exposure to ether. One group (180-220 g) showed a decline in pAVP of 27% at 2 min (P less than 0.05) and and 47% at 5 min (P less than 0.001) after stress. In a group of larger animals (350-400 g), pAVP levels were reduced by 55% at 1 min (P less than 0.05) and 72% at 2 min (P less than 0.01) after ether stress. A third group (250-300 g) also had significantly reduced pAVP values of 57% (P less than 0.01) 5 min after ether stress but not at 15 min. Nondehydrated rats which were centriguated at -4.1 Gx for 5, 15 or 120 min showed no significant alterations in pAVP. No decrease in pAVP was observed in dehydrated rats centrifugated for 5 min; after 120 min of centrifugation, mean pAVP was reduced by 40% (P less than 0.02) when compared to be noncentrifugated controls. In contrast to either ether or acceleration stress, nicotine provoked a marked rise (P less than 0.005) in pAVP 10 min after injection. From these results it was concluded that ether or acceleration stress does not evoke an increase in the pAVP levels of rats, and furthermore, in dehydrated rats, these stressors will produce a significant decline in pAVP.
本文描述了一种用于检测血浆精氨酸加压素（pAVP）的灵敏放射免疫测定法的开发。使用该测定法，测定了暴露于乙醚或加速应激后的未脱水和脱水大鼠血浆中的加压素水平。还测定了给予尼古丁后大鼠的血浆AVP。暴露于乙醚1分钟后1、2、5或15分钟，未脱水大鼠的pAVP无显著变化。另一方面，脱水大鼠暴露于乙醚后pAVP显著降低。一组（180 - 220克）在应激后2分钟时pAVP下降27%（P < 0.05），5分钟时下降47%（P < 0.001）。在一组较大的动物（350 - 400克）中，乙醚应激后1分钟时pAVP水平降低55%（P < 0.05），2分钟时降低72%（P < 0.01）。第三组（250 - 300克）在乙醚应激后5分钟时pAVP值也显著降低57%（P < 0.01），但15分钟时未降低。在-4.1 Gx离心5、15或120分钟的未脱水大鼠，其pAVP无显著变化。脱水大鼠离心5分钟时未观察到pAVP降低；与未离心的对照组相比，离心120分钟后，平均pAVP降低40%（P < 0.02）。与乙醚或加速应激不同，尼古丁注射后10分钟可引起pAVP显著升高（P < 0.005）。从这些结果得出结论，乙醚或加速应激不会引起大鼠pAVP水平升高，此外，在脱水大鼠中，这些应激源会导致pAVP显著下降。
