Chemistry Department, King Fahd University of Petroleum & Minerals, PO Box 6724, Dhahran 31261, Saudi Arabia.
Saudi Pharm J. 2012 Apr;20(2):155-60. doi: 10.1016/j.jsps.2011.08.005. Epub 2011 Sep 3.
A simple and fast-automated method was developed and validated for the assay of promethazine hydrochloride in pharmaceutical formulations, based on the oxidation of promethazine by cerium in an acidic medium. A portable system, consisting of a programmable syringe pump connected to a potentiometer, was constructed. The developed change in potential during promethazine oxidation was monitored. The related optimum working conditions, such as supporting electrolyte concentration, cerium(IV) concentration and flow rate were optimized. The proposed method was successfully applied to pharmaceutical samples as well as synthetic ones. The obtained results were realized by the official British pharmacopoeia (BP) method and comparable results were obtained. The obtained t-value indicates no significant differences between the results of the proposed and BP methods, with the advantages of the proposed method being simple, sensitive and cost effective.
建立并验证了一种基于酸性介质中铈（IV）氧化吩噻嗪的简单、自动化的盐酸异丙嗪制剂分析方法。构建了一个由可编程注射器泵与电位计相连的便携式系统，以监测吩噻嗪氧化过程中的电位变化。优化了相关最佳工作条件，如支持电解质浓度、铈（IV）浓度和流速。该方法成功应用于药物样品和合成样品。所得结果与英国药典（BP）法一致，且具有相似性。所得 t 值表明，所提出方法与 BP 法之间无显著差异，该方法具有简单、灵敏和经济有效的优点。
