Locatto M E, Abranzon H, Caferra D, Fernandez M C, Alloatti R, Puche R C
Laboratorio de Biologia Osea, Facultad de Ciencias Medicas, Rosario, Argentina.
Bone Miner. 1993 Nov;23(2):129-44. doi: 10.1016/s0169-6009(08)80049-9.
Body and skeletal growth and development were studied in alloxan-treated and age-matched control rats, between 3 and 23 weeks of age. For both groups the growth of the skeletal and body weights were in phase, with a maximum at 7 weeks of age. The growth data was assessed according to Parks' theory of feeding and growth. Alloxan-treated rats showed an important reduction in body and bone mass, with a greater impact on soft tissues. As expected, the asymptotic body and skeletal weights were reduced respect to controls. The time needed to attain 63% of mature food intake (Brody's 'time constant') was also reduced, indicating that maturation occurred at an earlier age than controls. The diabetic state is characterized by a reduced food conversion efficiency. Despite hyperfagia, alloxan-treated rats showed circa one-half the body and skeletal weights of age-matched controls. The following adverse effects of alloxan diabetes on bone tissue were observed: (a) a decrease in trabecular bone volume (femoral metaphyses) and cortical width (femoral diaphyses), (b) increased bone collagen glycosylation as a function of extracellular glucose concentration, (c) increased resistance of bone collagen to collagenase hydrolysis, (d) decreased rate of bone resorption except under strongly stimulated parathyroid function, (d) significantly lower ashes/bone matrix ratio in diabetic rats with more than 10 weeks of diabetes, and (e) no histological evidence of osteomalacia.
在3至23周龄的用四氧嘧啶处理的大鼠和年龄匹配的对照大鼠中研究了身体和骨骼的生长与发育。对于两组而言，骨骼和体重的增长是同步的，在7周龄时达到最大值。根据帕克斯的喂养和生长理论评估生长数据。用四氧嘧啶处理的大鼠的身体和骨量显著减少，对软组织的影响更大。正如预期的那样，与对照组相比，渐近身体和骨骼重量降低。达到成熟食物摄入量63%所需的时间（布罗迪的“时间常数”）也缩短了，这表明成熟发生的年龄比对照组更早。糖尿病状态的特征是食物转化效率降低。尽管食量过大，但用四氧嘧啶处理的大鼠的身体和骨骼重量约为年龄匹配对照组的一半。观察到四氧嘧啶糖尿病对骨组织有以下不良影响：(a) 小梁骨体积（股骨近端）和皮质宽度（股骨干）减少，(b) 骨胶原糖基化随细胞外葡萄糖浓度增加，(c) 骨胶原对胶原酶水解的抵抗力增加，(d) 除甲状旁腺功能受到强烈刺激外，骨吸收速率降低，(d) 糖尿病超过10周的糖尿病大鼠骨灰/骨基质比值显著降低，以及(e) 没有骨软化症的组织学证据。
