Zhang L, Hellström K E, Chen L
Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121.
Clin Exp Metastasis. 1994 Mar;12(2):87-92. doi: 10.1007/BF01753974.
The gene encoding firefly luciferase has been used as a reporter gene for the study of gene function. The luciferase catalyzes its substrate and subsequently produces luminescence. In addition, it is not present in mammalian cells. We have therefore explored its use in monitoring the growth of tumors in vivo. The luciferase gene was transfected into two murine tumor lines, i.e. c162 melanoma and M109 lung carcinoma, and the luciferase activity associated with the cells was determined by a rapid chemiluminescent reaction. Luciferase activity was well-correlated with the number of tumor cells in vitro. Luciferase activity also correlated with the tumor burden in vivo, as well as with the effect of an adoptive T cell transfer therapy in the syngeneic C3H/HeN mice experimental tumor model. This assay offers the advantage of being quantitative, rapid, and reliable for the detection of tumor burden and for the evaluation of the effect of antineoplastic therapy.
编码萤火虫荧光素酶的基因已被用作研究基因功能的报告基因。荧光素酶催化其底物并随后产生发光现象。此外，它不存在于哺乳动物细胞中。因此，我们探索了其在体内监测肿瘤生长方面的用途。将荧光素酶基因转染到两种小鼠肿瘤细胞系中，即c162黑色素瘤细胞系和M109肺癌细胞系，并通过快速化学发光反应测定与细胞相关的荧光素酶活性。荧光素酶活性在体外与肿瘤细胞数量高度相关。荧光素酶活性在体内也与肿瘤负荷相关，并且与同基因C3H/HeN小鼠实验性肿瘤模型中过继性T细胞转移疗法的效果相关。该检测方法具有定量、快速且可靠的优点，可用于检测肿瘤负荷以及评估抗肿瘤治疗的效果。
