秀丽隐杆线虫中决定寿命的基因：多个非相互作用位点的染色体定位
Longevity-determining genes in Caenorhabditis elegans: chromosomal mapping of multiple noninteractive loci.
作者信息
Ebert R H, Cherkasova V A, Dennis R A, Wu J H, Ruggles S, Perrin T E, Shmookler Reis R J
机构信息
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock 72205.
出版信息
Genetics. 1993 Dec;135(4):1003-10. doi: 10.1093/genetics/135.4.1003.
Abstract
We have used chromosome mapping with polymorphic markers to define genetic components governing life span in the nematode Caenorhabditis elegans. A complex recombinant-inbred population was derived from an interstrain cross, yielding > 1000 genotypes, each a composite of homozygous segments from the two parental strains. Genotypes were analyzed for the last-surviving 1-5% of worms in aging cohorts, and for young controls, by multiplex polymerase chain reaction using polymorphic markers to distinguish the parental alleles. We identified five regions of the genome at which one parental allele was significantly enriched in long-lived subpopulations. At four of five loci, the same alleles were selected in aging cohorts maintained under two different conditions, implying that these genes determine life span in differing environments.
摘要
我们利用多态性标记进行染色体图谱分析，以确定控制线虫秀丽隐杆线虫寿命的遗传成分。一个复杂的重组近交群体来自品系间杂交，产生了1000多个基因型，每个基因型都是两个亲本品系纯合片段的组合。通过使用多态性标记区分亲本等位基因的多重聚合酶链反应，对衰老群体中最后存活的1-5%的蠕虫以及年轻对照进行基因型分析。我们在基因组中确定了五个区域，其中一个亲本等位基因在长寿亚群中显著富集。在五个位点中的四个位点，在两种不同条件下维持的衰老群体中选择了相同的等位基因，这意味着这些基因在不同环境中决定寿命。
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