Love J M, Knight A M, McAleer M A, Todd J A
Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UK.
Nucleic Acids Res. 1990 Jul 25;18(14):4123-30. doi: 10.1093/nar/18.14.4123.
Fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis. 88% of the sequences, most of which contain the dinucleotide repeat, CA/GT, showed size variations between different inbred strains of mice and the wild mouse, Mus spretus. 62% of sequences had 3 or more alleles. GA/CT and AT/TA-containing sequences were also variable. About half of these size variants were detectable by agarose gel electrophoresis. This simple approach is extremely useful in linkage and genome mapping studies and will facilitate construction of high resolution maps of both the mouse and human genomes.
利用聚合酶链反应和凝胶电泳技术，对来自小鼠基因组数据库的50个包含简单序列重复或微卫星的序列进行了大小变异分析。88%的序列（其中大部分含有二核苷酸重复序列CA/GT）在不同近交系小鼠和野生小鼠（小家鼠）之间表现出大小变异。62%的序列有3个或更多等位基因。含GA/CT和AT/TA的序列也具有变异性。这些大小变异中的约一半可通过琼脂糖凝胶电泳检测到。这种简单的方法在连锁和基因组图谱研究中极其有用，将有助于构建小鼠和人类基因组的高分辨率图谱。
