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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Zhou Z X, Deng Z P, Ding J Y

College of Veterinary Medicine, Beijing Agricultural University, P. R. China.

Br Poult Sci. 1995 Mar;36(1):79-86. doi: 10.1080/00071669508417754.

The distribution of glycoconjugates on the surface of Salmonella pullorum and the ileal epithelium of chicks was demonstrated by lectin cytochemistry. The role of glycoconjugates in adherence of S. pullorum to the ileal epithelium was determined by a sugar inhibition assay using the scanning electron microscope. 2. S. pullorum exhibited binding to Concanavalin A (Con A) and wheat germ agglutinin (WGA) but not to soyabean agglutinin (SBA), Ricinus communis agglutinin (RCA) and Ulex europaeus agglutinin (UEA). 3. The ileal epithelium of chicks bound WGA, Con A and SBA. The binding sites for WGA were on the brush border and cytoplasm of columnar enterocytes as well as on goblet cells. The binding of Con A was confined to the cytoplasm of columnar enterocytes, while SBA bound, in a limited way, to the brush border of columnar enterocytes. 4. After an oral dose of S. pullorum, adherence to the ileal epithelium was inhibited by methyl alpha-D-mannopyranoside.

通过凝集素细胞化学方法证实了鸡白痢沙门氏菌表面和雏鸡回肠上皮细胞上糖缀合物的分布。利用扫描电子显微镜通过糖抑制试验确定了糖缀合物在鸡白痢沙门氏菌黏附于回肠上皮细胞中的作用。2. 鸡白痢沙门氏菌与刀豆球蛋白A（Con A）和麦胚凝集素（WGA）结合，但不与大豆凝集素（SBA）、蓖麻凝集素（RCA）和荆豆凝集素（UEA）结合。3. 雏鸡回肠上皮细胞与WGA、Con A和SBA结合。WGA的结合位点位于柱状肠上皮细胞的刷状缘和细胞质以及杯状细胞上。Con A的结合局限于柱状肠上皮细胞的细胞质，而SBA以有限的方式结合于柱状肠上皮细胞的刷状缘。4. 口服鸡白痢沙门氏菌后，α-D-吡喃甘露糖苷可抑制其对回肠上皮细胞的黏附。

Zhou Z X, Deng Z P, Ding J Y

College of Veterinary Medicine, Beijing Agricultural University, P. R. China.

Br Poult Sci. 1995 Mar;36(1):79-86. doi: 10.1080/00071669508417754.

The distribution of glycoconjugates on the surface of Salmonella pullorum and the ileal epithelium of chicks was demonstrated by lectin cytochemistry. The role of glycoconjugates in adherence of S. pullorum to the ileal epithelium was determined by a sugar inhibition assay using the scanning electron microscope. 2. S. pullorum exhibited binding to Concanavalin A (Con A) and wheat germ agglutinin (WGA) but not to soyabean agglutinin (SBA), Ricinus communis agglutinin (RCA) and Ulex europaeus agglutinin (UEA). 3. The ileal epithelium of chicks bound WGA, Con A and SBA. The binding sites for WGA were on the brush border and cytoplasm of columnar enterocytes as well as on goblet cells. The binding of Con A was confined to the cytoplasm of columnar enterocytes, while SBA bound, in a limited way, to the brush border of columnar enterocytes. 4. After an oral dose of S. pullorum, adherence to the ileal epithelium was inhibited by methyl alpha-D-mannopyranoside.

通过凝集素细胞化学方法证实了鸡白痢沙门氏菌表面和雏鸡回肠上皮细胞上糖缀合物的分布。利用扫描电子显微镜通过糖抑制试验确定了糖缀合物在鸡白痢沙门氏菌黏附于回肠上皮细胞中的作用。2. 鸡白痢沙门氏菌与刀豆球蛋白A（Con A）和麦胚凝集素（WGA）结合，但不与大豆凝集素（SBA）、蓖麻凝集素（RCA）和荆豆凝集素（UEA）结合。3. 雏鸡回肠上皮细胞与WGA、Con A和SBA结合。WGA的结合位点位于柱状肠上皮细胞的刷状缘和细胞质以及杯状细胞上。Con A的结合局限于柱状肠上皮细胞的细胞质，而SBA以有限的方式结合于柱状肠上皮细胞的刷状缘。4. 口服鸡白痢沙门氏菌后，α-D-吡喃甘露糖苷可抑制其对回肠上皮细胞的黏附。