非洲爪蟾卵母细胞中表达的神经元烟碱型受体α7的钡通透性。
Barium permeability of neuronal nicotinic receptor alpha 7 expressed in Xenopus oocytes.
作者信息
Sands S B, Costa A C, Patrick J W
机构信息
Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030.
出版信息
Biophys J. 1993 Dec;65(6):2614-21. doi: 10.1016/S0006-3495(93)81296-7.
Abstract
The rat alpha 7 neuronal nicotinic acetylcholine receptor was expressed and studied in Xenopus oocytes. The magnitude and reversal potential of instantaneous whole cell currents were examined in solutions containing varying concentrations of either calcium or barium, and in the presence or absence of the intracellular calcium chelator BAPTA. In external barium, application of nicotine elicits an inwardly rectifying response; in calcium the response is larger and has a linear IV relation. Pretreatment of oocytes with BAPTA-AM could not prevent activation of calcium-dependent chloride channels in external Ringer containing calcium. Using an extended GHK equation, the permeability ratio PBa/PNa of the alpha 7 receptor was determined to be about 17. Our results suggest that alpha 7 nicotinic receptors are highly permeable to divalent cations.
摘要
大鼠α7神经元烟碱型乙酰胆碱受体在非洲爪蟾卵母细胞中表达并进行了研究。在含有不同浓度钙或钡的溶液中，以及在存在或不存在细胞内钙螯合剂BAPTA的情况下，检测了瞬时全细胞电流的大小和反转电位。在外部钡溶液中，应用尼古丁会引发内向整流反应；在钙溶液中，反应更大且具有线性电流-电压关系。用BAPTA-AM预处理卵母细胞不能阻止在含钙的外部林格氏液中钙依赖性氯通道的激活。使用扩展的戈德曼-霍奇金-卡茨（GHK）方程，确定α7受体的通透率PB a/PNa约为17。我们的结果表明，α7烟碱型受体对二价阳离子具有高度通透性。
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