Altmann K G
CSIRO, Division of Animal Health, Armidale, NSW, Australia.
Immunol Cell Biol. 1993 Dec;71 ( Pt 6):517-25. doi: 10.1038/icb.1993.57.
The effect on anti-hapten immune responses of altering the isoelectric point of hapten-carrier conjugates by cationization was examined in sheep and mice. No adjuvants were employed in these studies. In sheep the cationization of ovalbumin-dinitrophenyl (OVA-DNP) resulted in an enhanced primary anti-dinitrophenyl (DNP) response. The secondary anti-DNP responses were not consistently greater for animals primed with cationized (CAT) OVA-DNP than in animals primed with OVA-DNP. Similar results were obtained with mice immunized with bovine serum albumin (BSA)-DNP or CAT BSA-DNP. Mice and sheep primed with OVA-DNP failed to mount an anamnestic response following secondary immunization with CAT OVA-DNP. Failure of a cationized hapten-carrier to stimulate a secondary anti-DNP response following priming with uncationized hapten-carrier was also observed in mice using BSA-DNP as the model antigen.
在绵羊和小鼠中检测了通过阳离子化改变半抗原-载体缀合物的等电点对抗半抗原免疫反应的影响。这些研究中未使用佐剂。在绵羊中，卵清蛋白-二硝基苯基（OVA-DNP）的阳离子化导致原发性抗二硝基苯基（DNP）反应增强。用阳离子化（CAT）OVA-DNP免疫的动物的继发性抗DNP反应并不总是比用OVA-DNP免疫的动物更强。用牛血清白蛋白（BSA）-DNP或CAT BSA-DNP免疫的小鼠也得到了类似的结果。用OVA-DNP免疫的小鼠和绵羊在用CAT OVA-DNP进行二次免疫后未能产生回忆反应。在小鼠中，以BSA-DNP作为模型抗原，也观察到用未阳离子化的半抗原-载体免疫后，阳离子化的半抗原-载体未能刺激继发性抗DNP反应。
