来自人胰岛cDNA文库的新型表达序列标签的染色体定位和组织分布

Chromosomal assignment and tissue distribution of novel expressed sequence tags from a human pancreatic islet cDNA library.

作者信息

Takeda J, Espinosa R, Eng S, Le Beau M M, Bell G I

机构信息

Department of Medicine, University of Chicago, Illinois 60637, USA.

出版信息

Genomics. 1995 Sep 1;29(1):276-81. doi: 10.1006/geno.1995.1245.

DOI:10.1006/geno.1995.1245

PMID:8530086

Abstract

One hundred novel cDNAs from a human pancreatic islet library have been mapped using PCR and DNA from a human-rodent somatic cell hybrid mapping panel and/or fluorescence in situ hybridization. In addition, the pattern of expression of 54 of these cDNAs has been determined by RNA blotting.

摘要

利用聚合酶链反应（PCR）以及来自人-啮齿动物体细胞杂交定位板的DNA和/或荧光原位杂交技术，已对来自人胰岛文库的100个新的互补DNA（cDNA）进行了定位。此外，通过RNA印迹法确定了其中54个cDNA的表达模式。