通过荧光原位杂交将20个单拷贝基因座亚定位到6号染色体上。
Subregional localization of 20 single-copy loci to chromosome 6 by fluorescence in situ hybridization.
作者信息
Rao P H, Murty V V, Gaidano G, Hauptschein R, Dalla-Favera R, Chaganti R S
机构信息
Laboratory of Cancer Genetics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
出版信息
Genomics. 1993 May;16(2):426-30. doi: 10.1006/geno.1993.1206.
Although 338 genetic loci and 1 or more candidate tumor suppressor genes have been assigned to chromosome 6, the physical and genetic map of this chromosome is at a very preliminary stage. In this study, we have performed subregional localization of 20 single-copy DNA sequences previously assigned to chromosome 6 using the fluorescence in situ hybridization technique. One locus, D6S6, was localized at 6p12. Two loci, D6S160 and D6S32, were localized in proximal 6q, at 6q12 and 6q23.3, respectively. The remaining loci were clustered in two regions, 4 at 6q23.5-q25 (D6S33, D6S43, D6S85, MYB) and 13 at 6q26-q27 (D6S2, D6S21, D6S22, D6S25, D6S37, D6S39, D6S44, D6S48, D6S132, D6S133, D6S148, D6S170, D6S201). These data will aid in the eventual development of the physical map of chromosome 6.
尽管已有338个基因位点以及1个或更多的候选肿瘤抑制基因被定位到6号染色体上，但该染色体的物理图谱和遗传图谱仍处于非常初步的阶段。在本研究中，我们利用荧光原位杂交技术对先前定位到6号染色体上的20个单拷贝DNA序列进行了亚区域定位。一个位点D6S6定位于6p12。两个位点D6S160和D6S32分别定位于6号染色体长臂近端的6q12和6q23.3。其余位点聚集在两个区域，4个位于6q23.5 - q25（D6S33、D6S43、D6S85、MYB），13个位于6q26 - q27（D6S2、D6S21、D6S22、D6S25、D6S37、D6S39、D6S44、D6S48、D6S132、D6S133、D6S148、D6S170、D6S201）。这些数据将有助于最终构建6号染色体的物理图谱。
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